We believe this site might serve you best::

United States

United States

Choose language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

RNase ONE™ Ribonuclease

Degrades RNA to Cyclic Nucleotide Monophosphate (NMP) Intermediates

  • Ability to cleave a phosphodiester bond between any two ribonucleotides

Size

Catalog number selected: M4261

$ 114.00 Your price: Log In

Add to Helix Add to Helix
RNase ONE™ Ribonuclease
1,000u
$ 114.00
Your price: Log In
Change Configuration

RNase ONE™ Ribonuclease is a 27kDa periplasmic enzyme from E. coli that catalyzes the degradation of RNA to cyclic nucleotide monophosphate (NMP) intermediates. Slower hydrolysis further catalyzes the degradation of these intermediates to 3´-NMPs. RNase ONE™ Ribonuclease may be used to remove RNA from DNA preparations, for mismatch detection, RNase protection assays and mapping, or quantitation of RNA by selective cleavage of single-stranded regions. The enzyme is provided with 10X Reaction Buffer: 100mM Tris-HCl (pH 7.5 at 25°C), 50mM EDTA, 2M sodium acetate.

References
  1. Shen, V. and Schlessinger, D. (1982) The Enzymes, Vol. XV, 501.
  2. Meador, J., 3rd et al. (1990) Eur. J. Biochem. 187, 549–53.
  3. Meador, J., 3rd and Kennell, D. (1990) Gene 95, 1–7.

Storage Buffer: 10mM Tris-HCl (pH 8.0), 200mM NaCl, 50% (v/v) glycerol.

Source: Recombinant E. coli strain.

QC Tests: Activity, SDS-PAGE/purity, endonuclease, DNase.

Unit Definition: One unit is defined as the amount of enzyme required tocompletely degrade RNA at the rate of 100ng/second at 37°C in 10mM Tris-HCl (pH 7.5 at 25°C), 5mM EDTA, 200mM sodium acetate, 0.2μg/μl RNA, 0.05% NP-40 and 2μg/μl BSA.

Specifications

You are viewing: 1,000u Change Configuration

What's in the box?

Item Part # Size

RNase ONE™ 10X Reaction Buffer

M217A 1 x 1ml

RNase ONE™ Ribonuclease

M426A 1 x 1,000u

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB

See Protocol for storage recommendations.

Specifications

You are viewing: 5,000u Change Configuration

What's in the box?

Item Part # Size

RNase ONE™ 10X Reaction Buffer

M217A 5 x 1ml

RNase ONE™ Ribonuclease

M426C 1 x 5,000u

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB

See Protocol for storage recommendations.

Resources

Choose your country

Americas

Brazil
Brazil
Canada
Canada
United States
United States

Pacific Asia

Australia
Australia
India
India
Japan
Japan
Korea, Republic of
Korea, Republic of
Singapore
Singapore

Europe

Austria
Austria
Belgium
Belgium
Denmark
Denmark
Estonia
Estonia
Finland
Finland
France
France
Germany
Germany
Iceland
Iceland
Italy
Italy
Luxembourg
Luxembourg
Netherlands
Netherlands
Norway
Norway
Poland
Poland
Spain
Spain
Sweden
Sweden
Switzerland
Switzerland
United Kingdom
United Kingdom