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RNase ONE™ Ribonuclease

M4261_RNase-ONE--Ribonuclease--1-000u_3
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Degrades RNA to Cyclic Nucleotide Monophosphate (NMP) Intermediates

  • Ability to cleave a phosphodiester bond between any two ribonucleotides
  • Supplied at a concentration of 5–10u/μl

Size

Catalog number selected: M4261

$ 116.00
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RNase ONE™ Ribonuclease
1,000u
$ 116.00
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RNase ONE™ Ribonuclease is a 27kDa periplasmic enzyme from E. coli that catalyzes the degradation of RNA to cyclic nucleotide monophosphate (NMP) intermediates. Slower hydrolysis further catalyzes the degradation of these intermediates to 3´-NMPs. RNase ONE™ Ribonuclease may be used to remove RNA from DNA preparations, for mismatch detection, RNase protection assays and mapping, or quantitation of RNA by selective cleavage of single-stranded regions. The enzyme is provided with 10X Reaction Buffer: 100mM Tris-HCl (pH 7.5 at 25°C), 50mM EDTA, 2M sodium acetate.

References
  1. Shen, V. and Schlessinger, D. (1982) The Enzymes, Vol. XV, 501.
  2. Meador, J., 3rd et al. (1990) Eur. J. Biochem. 187, 549–53.
  3. Meador, J., 3rd and Kennell, D. (1990) Gene 95, 1–7.

Storage Buffer: 10mM Tris-HCl (pH 8.0), 200mM NaCl, 50% (v/v) glycerol.

Source: Recombinant E. coli strain.

QC Tests: Activity, SDS-PAGE/purity, endonuclease, DNase.

Unit Definition: One unit is defined as the amount of enzyme required tocompletely degrade RNA at the rate of 100ng/second at 37°C in 10mM Tris-HCl (pH 7.5 at 25°C), 5mM EDTA, 200mM sodium acetate, 0.2μg/μl RNA, 0.05% NP-40 and 2μg/μl BSA.

Specifications

You are viewing: M4261 Change Configuration

What's in the box?

Item Part # Size

RNase ONE™ 10X Reaction Buffer

M217A 1 × 1ml

RNase ONE™ Ribonuclease

M426A 1 × 1,000u

SDS

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Download SDSPDF (42 KB) – English (United States)

Certificate of Analysis

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Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB

See Protocol for storage recommendations.

Specifications

You are viewing: M4265 Change Configuration

What's in the box?

Item Part # Size

RNase ONE™ 10X Reaction Buffer

M217A 5 × 1ml

RNase ONE™ Ribonuclease

M426C 1 × 5,000u

SDS

Choose language:

Download SDSPDF (42 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB

See Protocol for storage recommendations.

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