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Promega Corporation

FluoroTect™ GreenLys in vitro Translation Labeling System

The FluoroTect™ GreenLys in vitro Translation Labeling System allows for the fluorescent labeling and detection of proteins synthesized in vitro. The system is based on a lysine-charged tRNA that is labeled at the ε position of the lysine with the fluorophore BODIPY®-FL. Fluorescent lysine residues will be incorporated into synthesized proteins during in vitro translation reactions, eliminating the need for radioactivity.

Detection of the labeled proteins is accomplished in 2–5 minutes directly "in-gel" by use of a laser-based fluorescent gel scanner. This eliminates any requirements for protein gel manipulatio...

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FluoroTect™ GreenLys in vitro Translation Labeling System

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FluoroTect™ GreenLys in vitro Translation Labeling System

 Components

  • FluoroTect™ GreenLys tRNA

    L500A1 x 40μl
40 reactions
- L5001 $ 350.00 Add to cart


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Storage Conditions

Store at –70°C.

For product intended use please see Patents & Disclaimers tab.

Schematic illustrating the incorporation of FluoroTect™ Green<sub>Lys</sub>-labeled lysine into nascent protein.Figure 1. Schematic illustrating the incorporation of FluoroTect™ GreenLys-labeled lysine into nascent protein.

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Use Restrictions

L5001 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

L5001 FluoroTect™ GreenLys incorporates Fluorotag® technology, which is licensed under U.S. Pat. Nos. 6,306,628 and 7,252,932. Commercial use of this product requires a license from AmberGen, Inc. Fluorotag is a registered trademark of AmberGen, Inc.

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