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GoScript™ Reverse Transcriptase utilizes M-MLV and state-of-the-art buffer technology designed for qPCR to deliver robust, reliable cDNA synthesis of a full range of rare and abundant transcripts, even in the presence of inhibitors. GoScript™ Reverse Transcriptase is qualified for use in qPCR, including GoTaq® qPCR and Plexor® qPCR systems for performing RT-qPCR.
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GoScript™ Reverse Transcriptase
GoScript™ 5X Reaction Buffer
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This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.
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Store at –20°C.
For product intended use please see Patents & Disclaimers tab.
Human colon total RNA (Clontech Laboratories, Inc.) was reverse transcribed using oligo(dT) (O) or random primers (R) according to the manufacturer’s instructions for each reverse transcriptase. cDNA then was analyzed by endpoint PCR using GoTaq® Green Master Mix (Cat.# M7122) with primers for a 940bp amplicon at the 5´ end of the transcript. Each reaction (5µl) was analyzed by agarose gel electrophoresis and ethidium bromide staining. All samples shown were analyzed in the same data set and gel with the same scan settings. Gel images are representative of results from three separate experiments.
A5003, A5004 For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.
A5003, A5004 U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.
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