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Promega Corporation

T4 DNA Ligase

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T4 DNA Ligase catalyzes the joining of two strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides in either a cohesive-ended or blunt-ended configuration. The enzyme has also been shown to catalyze the joining of RNA to either a DNA or RNA strand in a duplex molecule but will not join single-stranded nucleic acids.

The T4 DNA Ligase Buffer Pack includes 3 tubes of T4 DNA Ligase 10X Reaction Buffer. The composition of the 10X reaction buffer is 300mM Tris-HCl (pH 7.8 at 25°C), 100mM MgCl2, 100mM DTT and 10mM ATP.

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  • Prices valid for US customers only
Product Size Conc. Catalog # *List Price QTY Add to Cart

T4 DNA Ligase

100u
(Weiss units)
1–3u/μl M1801 $ 48.00 Add to cart

T4 DNA Ligase

500u
(Weiss units)
1–3u/μl M1804 $ 190.00 Add to cart

T4 DNA Ligase (HC)

500u
(Weiss units)
10–20u/μl M1794 $ 195.00 Add to cart


Available Separately

Product Size Conc. Catalog # *List Price QTY Add to Cart

T4 DNA Ligase Buffer Pack

1.5ml
(3 × 500μl)
- C1263 $ 18.00 Add to cart

QC Tests

Activity, SDS-PAGE/purity, DNase, endonuclease, RNase, blue/white cloning assay.

Source

Recombinant E. coli strain.

Storage Buffer

10mM Tris-HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA and 50% glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

0.01 Weiss unit of T4 DNA Ligase is the amount of enzyme required to catalyze the ligation of greater than 95% of 1μg of λ/HindIII fragments at 16°C in 20 minutes.

For product intended use please see Patents & Disclaimers tab.

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