Kinase Enzyme Systems Technical Manual
Instructions for Use of Product(s)
V1901, V1911, V1921, V1931, V1941, V1951, V1961, V1971, V1981, V1991, V2621, V2681, V2691, V2701, V2741, V2761, V2781, V2841, V2911, V2921, V2931, V2941, V2951, V2961, V2971, V2981, V2991, V3001, V3051, V3101, V3191, V3201, V3231, V3271, V3361, V3371, V3381, V3391, V3401, V3411, V3501, V3521, V3531, V3541, V3561, V3571, V3581, V3701, V3711, V3721, V3731, V3741, V3751, V3761, V3801, V3811, V3821, V3891, V3892, V4024, V4026, V4028, V4030, V4032, V4034, V4036, V4038, V4040, V4042, V4044, V4046, V4048, V4050, V4052, V4054, V4056, V4058, V4060, V4062, V4064, V9381
Literature # TM553
The ADP-Glo™ Kinase Assay (Cat.# V6930, V9101, V9102) is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is converted into light by Ultra-Glo™ Luciferase. The luminescent signal positively correlates with kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. The ADP-Glo™ Max Assay (Cat.# V7001) can be used when higher concentrations (up to 5mM) are required.
Summary of Changes
The following changes were made to the 4/22 revision of this document:
1. In Section 3.B, Step 1, the percentage of DMSO was updated to 5%.
2. Typos were corrected.
3. The cover image was updated.
Revised 4/22.
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Product Categories
Products
- ABL1 Kinase Enzyme System
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- AKT1 Kinase Enzyme System
- ALK6 Kinase Enzyme System
- AMPK (A1/B1/G1) Kinase Enzyme System
- Aurora A Kinase Enzyme System
- BRK Kinase Enzyme System
- BTK Kinase Enzyme System
- CAMK2γ Kinase Enzyme System
- CAMK4 Kinase Enzyme System
- CDK1/CyclinA2 Kinase Enzyme System
- CDK2/CyclinA2 Kinase Enzyme System
- CDK5/p25 Kinase Enzyme System
- CDK5/p35 Kinase Enzyme System
- CHK1 Kinase Enzyme System
- CLK1 Kinase Enzyme System
- c-MER Kinase Enzyme System
- CSK Kinase Enzyme System
- DDR2 Kinase Enzyme System
- EPHA1 Kinase Enzyme System
- ERK1 Kinase Enzyme System
- ERK2 Kinase Enzyme System
- FAK Kinase Enzyme System
- FES Kinase Enzyme System
- FGFR1 Kinase Enzyme System
- FGFR2 Kinase Enzyme System
- FGFR4 Kinase Enzyme System
- FLT1 Kinase Enzyme System
- FLT3 Kinase Enzyme System
- FYN A Kinase Enzyme System
- GSK3α Kinase Enzyme System
- GSK3β Kinase Enzyme System
- HER2 Kinase Enzyme System
- HER4 Kinase Enzyme System
- IGF1R Kinase Enzyme System
- IRAK4 Kinase Enzyme System
- ITK Kinase Enzyme System
- JAK3 Kinase Enzyme System
- JNK3 Kinase Enzyme System
- KDR Kinase Enzyme System
- LCK Kinase Enzyme System
- LYN B Kinase Enzyme System
- MAPKAPK2 Kinase Enzyme System
- MAPKAPK3 Kinase Enzyme System
- MARK1 Kinase Enzyme System
- MET Kinase Enzyme System
- p38α Kinase Enzyme System
- p38γ Kinase Enzyme System
- p70S6K Kinase Enzyme System
- p70S6Kb Kinase Enzyme System
- PAK4 Kinase Enzyme System
- PDGFRα Kinase Enzyme System
- PDGFRβ Kinase Enzyme System
- PDK1 Kinase Enzyme System
- PIM1 Kinase Enzyme System
- PIM2 Kinase Enzyme System
- PKCα Kinase Enzyme System
- PKCβII Kinase Enzyme System
- PKCγ Kinase Enzyme System
- PKCδ Kinase Enzyme System
- PKCε Kinase Enzyme System
- PKCζ Kinase Enzyme System
- PKCθ Kinase Enzyme System
- PKCι Kinase Enzyme System
- PKCμ Kinase Enzyme System
- PKD2 Kinase Enzyme System
- PLK1 Kinase Enzyme System
- RET Kinase Enzyme System
- ROCK1 Kinase Enzyme System
- ROCK2 Kinase Enzyme System
- RSK1 Kinase Enzyme System
- RSK2 Kinase Enzyme System
- SGK1 Kinase Enzyme System
- SRC Kinase Enzyme System
- SYK Kinase Enzyme System
- TRKA Kinase Enzyme System
- TRKB Kinase Enzyme System
- ULK1 Kinase Enzyme System
- ZAP70 Kinase Enzyme System