Compatibility of Single Step (KRX) Competent Cells with the MagneGST™ Pull-Down System

Trista Schagat
Promega Corporation
Publication Date: 2007


This study demonstrates the use of Single Step (KRX) Competent Cells for studying protein:protein interactions using the MagneGST™ Pull-Down System. GST-fusion bait protein expressed in KRX was captured on MagneGST™ glutathione-linked magnetic particles and incubated with prey protein expressed in vitro in the TnT® T7 Quick Coupled Transcription/Translation System. Captured prey was detected using HaloTag® Interchangeable Labeling Technology.

Promega Notes 96, 22–23.