Malate-Glo™ Assay Technical Manual
Instructions for Use of Product(s)
JE9100, JE9200
Literature # TM696
The Malate-Glo™ Assay is a bioluminescent assay for rapid, selective and sensitive detection of L-malate in biological samples. L-malate is a tricarboxylic acid cycle (TCA) intermediate and a critical component of the malate-aspartate shuttle. In plants, malate plays an important role in the Calvin cycle. The Malate-Glo™ Assay couples malate oxidation and NADH production with a bioluminescent NADH detection system. When malate detection reagent is added to a sample at a 1:1 ratio, the coupled-enzyme reactions start and run simultaneously. The luminescent signal is proportional to the amount of malate in the sample and increases until all malate is consumed, at which point a stable luminescent signal is achieved.
Summary of Changes
The following changes have been made to the 7/24 revision of this document:
1. In Section 5.A, two Thermo Fisher Scientific catalog numbers were updated.
2. In Section 5.A, formatting of the sensitivity cell number was changed.
3. Third party trademarks were added or updated.
Revised 7/24.