GoTaq® Master Mixes
All-in-One Optimized PCR Master Mix—Just Add Template, Primers and Water
- Directly substitute into your current PCR conditions
- Use the green master mix for direct-to-gel analysis after amplification
- Use the colorless master mix for post-amplification analysis by fluorescence or absorbance without prior DNA purification
Catalog Number:
Choose a master mix
Size
Catalog Number: M7122
Catalog Number: M7123
Catalog Number: M7132
Catalog Number: M7133
Set Up PCR in Less than a Minute with Convenient Master Mixes
GoTaq® DNA Polymerase is a proprietary formulation of Taq DNA polymerase that gives robust amplification equal to and, in some cases, superior to that of standard Taq DNA polymerase.
For ultimate in convenience, choose GoTaq® Colorless Master Mix or GoTaq® Green Master Mix and directly substitute in your current PCR application—no need to change cycling parameters.
Both Master Mixes are ready-to-use 2X solutions that contain GoTaq® DNA Polymerase, dNTPs, MgCl2 and reaction buffer at optimal concentrations for efficient amplification of DNA templates by PCR. GoTaq® Green Master Mix also includes two dyes (blue and yellow) that allow monitoring of progress during electrophoresis. The blue dye comigrates at the same rate as 3–5kb DNA fragments in a 1% agarose gel. The yellow dye migrates ahead of primers (<50bp).
GoTaq® Colorless Master Mix has the same formulation as the GoTaq® Green Master Mix but does not include the dyes. Both also include Nuclease-Free Water. Reactions assembled with the GoTaq® Master Mixes have sufficient density for direct loading onto agarose gels.
Reactions can be set up in less than a minute; simply add your template, water and primers and go!
GoTaq® Green and Colorless Master Mix reaction size is based on a 50µl reaction volume.Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
GoTaq® Green Master Mix, 2X |
M712B | 2 × 1,250μl | |
|
Nuclease-Free Water |
P119A | 2 × 1,250μl | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
GoTaq® Green Master Mix, 2X |
M712C | 1 × 25ml | |
|
Nuclease-Free Water |
P119C | 1 × 25ml | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
GoTaq® Colorless Master Mix, 2X |
M713B | 2 × 1,250μl | View Product |
|
Nuclease-Free Water |
P119A | 2 × 1,250μl | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
GoTaq® Colorless Master Mix, 2X |
M713C | 1 × 25ml | View Product |
|
Nuclease-Free Water |
P119C | 1 × 25ml | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Condiciones de Almacenaje
Related Products
Productos Similares
GoTaq® G2 Master Mixes
GoTaq® G2 DNA Polymerase de segunda generación y alto rendimiento en una master mix lista para su uso.
M7822, M7823, M7832, M7833
GoTaq® G2 Hot Start Master Mixes
Master mix optimizada lista para su uso en la preparación de PCR a temperatura ambiente. Contiene la enzima GoTaq® G2.
M7422, M7423, M7432, M7433
GoTaq® Hot Start Master Mixes
Master mix de PCR lista para su uso en la preparación de reacciones a temperatura ambiente.
M5122, M5123, M5132, M5133
GoTaq® Long PCR Master Mix
Master mix hot-start ideal para Long PCR.
M4021
Usado con frecuencia junto con
ReliaPrep™ Blood gDNA Miniprep System
Purificación rápida y manual de ADN de alta calidad a partir de muestras de sangre o fluidos corporales de hasta 200μl.
A5081, A5082
ReliaPrep™ gDNA Tissue Miniprep System
Purificación manual de ADN a partir de 25mg de tejido, 1 hisopo bucal o 1cm de cola de ratón.
A2051, A2052
Wizard® SV Gel and PCR Clean-Up System
Purifica fragmentos de ADN a partir de secciones de gel o de reacciones PCR, en tan sólo 15 minutos.
A9281, A9282, A9285, A9283, A9284
