We believe this site might serve you best:

United States

English Continue

This country code will remain if no action is taken to change it.

Don't see your country?
Promega Corporation

Improve Protein Analysis with the New Mass Spectrometry-Compatible ProteaseMax™ Surfactant

  • Print
  • Email
  • Download PDF


Incomplete solubilization and digestion and poor peptide recovery are frequent limitations in protein sample preparation for mass spectrometry (MS) analysis. Additives such as SDS or urea can improve protein solubilization and denaturation, but they tend to have negative effects on digestion and MS analysis. Here we present a novel acid- and thermo-labile surfactant, ProteaseMax™ Surfactant, that improves solubilization, digestion and peptide recovery while avoiding negative effects observed with common solubilizing agents. The surfactant is designed to degrade over the course of a typical sample preparation protocol, generating zwitterionic and neutral species that do not interfere with MS.

Promega Notes 99, 3–7.

Sergei Saveliev1, Daniel Simpson1, William Daily2, Carolyn Woodroofe2, Dieter Klaubert2, Grzegorz Sabat3, Robert Bulliet1, and Keith V. Wood1

1Promega Corporation, 2Promega Biosciences, 3Mass Spectrometry Facility, Biotechnology Center, University of Wisconsin-Madison.
Publication Date: 2008

Download Article (412 KB)

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.

Scientists at Your Service

Scientists at Your Service

We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.

Ask us! We are here to help you.