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Promega Corporation

QuantiFluor® dsDNA System Technical Manual

  • Instructions for Use of Product(s)
  • E2670

Literature # TM346

The QuantiFluor® dsDNA System contains a fluorescent DNA-binding dye (504nmEx/531nmEm) that enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA). This dye shows minimal binding to single-stranded DNA (ssDNA) and RNA, allowing specific quantitation of dsDNA.

The QuantiFluor® dsDNA Dye was developed using the fluorescence module of GloMax® Multi+ Detection System and the QuantiFluor®-ST Handheld Fluorometer. The Quantus™ Fluorometer was optimized for use with the QuantiFluor® Dyes, including the QuantiFluor® dsDNA Dye. The QuantiFluor® dsDNA System can be used with any fluorometer that is capable of measuring fluorescence at the appropriate excitation and emission wavelengths.

Summary of Changes: 2/15 Revision
The following changes were made to the 2/15 version of this document:

The calculation for the DNA concentration of the unknown samples was updated to remove the instructions to multiple the value by 2.

Printed in USA. Revised 2/15.


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