QuantiFluor® dsDNA System Technical Manual
Literature # TM346
The QuantiFluor® dsDNA System contains a fluorescent DNA-binding dye (504nmEx/531nmEm) that enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA). This dye shows minimal binding to single-stranded DNA (ssDNA) and RNA, allowing specific quantitation of dsDNA.
The QuantiFluor® dsDNA Dye was developed using the fluorescence module of GloMax® Multi+ Detection System and the QuantiFluor®-ST Handheld Fluorometer. The Quantus™ Fluorometer was optimized for use with the QuantiFluor® Dyes, including the QuantiFluor® dsDNA Dye. The QuantiFluor® dsDNA System can be used with any fluorometer that is capable of measuring fluorescence at the appropriate excitation and emission wavelengths.
Summary of Changes: 2/15 Revision
The following changes were made to the 2/15 version of this document:
The calculation for the DNA concentration of the unknown samples was updated to remove the instructions to multiple the value by 2.
Printed in USA. Revised 2/15.