QuantiFluor® dsDNA System Technical Manual
Literature # TM346
The QuantiFluor® dsDNA System contains a fluorescent DNA-binding dye (504nmEx/531nmEm) that enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA). This dye shows minimal binding to single-stranded DNA (ssDNA) and RNA, allowing specific quantitation of dsDNA.
The QuantiFluor® dsDNA Dye was developed using the fluorescence module of GloMax® Multi+ Detection System and the QuantiFluor®-ST Handheld Fluorometer. The Quantus™ Fluorometer was optimized for use with the QuantiFluor® Dyes, including the QuantiFluor® dsDNA Dye. The QuantiFluor® dsDNA System can be used with any fluorometer that is capable of measuring fluorescence at the appropriate excitation and emission wavelengths.
Summary of Changes: 5/14 Revision
The following changes were made to the 5/14 version of this document:
Storage conditions were updated in Section 2 and information regarding handling Lambda DNA Standard, 100μg/ml, was added to Sections 2, 4 and 7.
Printed in USA. Revised 5/14.