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Promega Corporation

HaloTag® Mammalian Protein Detection and Purification Systems Technical Manual

Literature # TM348

HaloTag® is a protein fusion tag engineered to form a highly specific, covalent bond with HaloLink™ Resin. This covalent binding coupled with the low nonspecific binding of the HaloLink™ Resin provide superior purity and recovery of recombinant proteins from cultured mammalian cells, even at low expression levels. The HaloTag® Mammalian Protein Detection and Purification System (Cat.# G6795) also includes the HaloTag® TMRDirect™ Ligand, which allows simple fluorescent detection of HaloTag® fusion proteins for rapid optimization of expression and purification conditions.

Summary of Changes
In the 9/14 revision of this document, the amount of HaloTEV Protease supplied with the HaloTag® Mammalian Protein Detection and Purification System was changed to specify volume (200μl) rather than units, and the storage temperature for HaloTEV Protease was changed to "below –65°C".

Printed in USA. Revised 9/14.


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