We believe this site might serve you best:

United States

English Continue

This country code will remain if no action is taken to change it.

Don't see your country?
Promega Corporation

HaloTag® Mammalian Protein Detection and Purification Systems Technical Manual

Literature # TM348

HaloTag® is a protein fusion tag engineered to form a highly specific, covalent bond with HaloLink™ Resin. This covalent binding coupled with the low nonspecific binding of the HaloLink™ Resin provide superior purity and recovery of recombinant proteins from cultured mammalian cells, even at low expression levels. The HaloTag® Mammalian Protein Detection and Purification System (Cat.# G6795) also includes the HaloTag® TMRDirect™ Ligand, which allows simple fluorescent detection of HaloTag® fusion proteins for rapid optimization of expression and purification conditions.

Summary of Changes
In the 9/14 revision of this document, the amount of HaloTEV Protease supplied with the HaloTag® Mammalian Protein Detection and Purification System was changed to specify volume (200μl) rather than units, and the storage temperature for HaloTEV Protease was changed to "below –65°C".

Printed in USA. Revised 9/14.

 

PDF Icon Complete Protocol PDF (1467kb)

PDF Icon Quick Protocol PDF (137kb)

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.

Scientists at Your Service

Scientists at Your Service

We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.

Ask us! We are here to help you.