We believe this site might serve you best:

United States

English Continue

This country code will remain if no action is taken to change it.

Don't see your country?
Promega Corporation

Calpain-Glo™ Protease Assay Technical Bulletin

Literature # TB344

The Calpain-Glo™ Protease Assay is a homogeneous, luminescent assay that measures calpain I (μ) and II (m) activities. The Calpain-Glo™ Protease Assay provides a succinyl proluminescent calpain substrate, Suc-LLVY-aminoluciferin, in a buffer system optimized for calpain activity and luciferase activity. Adding the single Calpain-Glo™ Reagent to test samples in an “add-mix-measure” format results in calpain cleavage of the substrate and generation of a “glow-type” luminescent signal produced by the luciferase reaction. In this homogeneous, coupled-enzyme format, the signal is proportional to the amount of calpain activity present. The calpain and luciferase enzyme activities reach a steady-state so that the luminescent signal peaks rapidly and achieves maximum sensitivity in 5–15 minutes. The homogeneous Calpain-Glo™ Assay is designed for use with multiwell plate formats, making it ideal for automated high-throughput screening (HTS) of calpain activity and inhibition.

Printed in USA. Revised 10/11.


PDF Icon Complete Protocol PDF (1146kb)

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.

Scientists at Your Service

Scientists at Your Service

We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.

Ask us! We are here to help you.