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Promega Corporation

HaloTag® Fusion (C-Terminal) Mammalian Expression Vectors

See All HaloTag® Vectors
HaloTag® Fusion (N-Terminal) Mammalian Expression Vectors
Inducible T7-Driven Flexi® Vectors for E. coli Expression

These vectors are designed for expression of C-terminal-tagged HaloTag® fusion proteins in mammalian cells. Once expressed, the HaloTag® fusion protein may be used for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag® Ligands. In addition, the HaloTag® fusion protein can be purified or pulled down as a complex with its protein partners.

We offer two types of HaloTag® fusion ve...

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Product Size Conc. Catalog # *List Price Order QTY Add to Cart

pHTC HaloTag® CMV-neo Vector

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pHTC HaloTag® CMV-neo Vector

 Components

  • pHTC HaloTag® CMV-neo Vector

    G771A1 x 20μg
20μg
- G7711 $ 205.00 Add to cart

pFC27A HaloTag® CMV-neo Flexi® Vector

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pFC27A HaloTag® CMV-neo Flexi® Vector

 Components

  • pFC27A HaloTag® CMV-neo Flexi® Vector

    G842A1 x 20μg
20μg
- G8421 $ 224.00 Add to cart

pFC27K HaloTag® CMV-neo Flexi® Vector

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pFC27K HaloTag® CMV-neo Flexi® Vector

 Components

  • pFC27K HaloTag® CMV-neo Flexi® Vector

    G843A1 x 20μg
20μg
- G8431 $ 224.00 Add to cart

pFC14A HaloTag® CMV Flexi® Vector

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pFC14A HaloTag® CMV Flexi® Vector

 Components

  • pFC14A HaloTag® CMV Flexi® Vector

    G965A1 x 20μg
20μg
- G9651 $ 224.00 Add to cart

pFC14K HaloTag® CMV Flexi® Vector

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pFC14K HaloTag® CMV Flexi® Vector

 Components

  • pFC14K HaloTag® CMV Flexi® Vector

    G966A1 x 20μg
20μg
- G9661 $ 224.00 Add to cart

pFC15A HaloTag® CMVd1 Flexi® Vector

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pFC15A HaloTag® CMVd1 Flexi® Vector

 Components

  • pFC15A HaloTag® CMVd1 Flexi® Vector

    G161A1 x 20μg
20μg
- G1611 $ 224.00 Add to cart

pFC15K HaloTag® CMVd1 Flexi® Vector

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pFC15K HaloTag® CMVd1 Flexi® Vector

 Components

  • pFC15K HaloTag® CMVd1 Flexi® Vector

    G160A1 x 20μg
20μg
- G1601 $ 224.00 Add to cart

pFC16A HaloTag® CMVd2 Flexi® Vector

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pFC16A HaloTag® CMVd2 Flexi® Vector

 Components

  • pFC16A HaloTag® CMVd2 Flexi® Vector

    G159A1 x 20μg
20μg
- G1591 $ 224.00 Add to cart

pFC16K HaloTag® CMVd2 Flexi® Vector

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pFC16K HaloTag® CMVd2 Flexi® Vector

 Components

  • pFC16K HaloTag® CMVd2 Flexi® Vector

    G157A1 x 20μg
20μg
- G1571 $ 224.00 Add to cart

pFC17A HaloTag® CMVd3 Flexi® Vector

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pFC17A HaloTag® CMVd3 Flexi® Vector

 Components

  • pFC17A HaloTag® CMVd3 Flexi® Vector

    G155A1 x 20μg
20μg
- G1551 $ 224.00 Add to cart

pFC17K HaloTag® CMVd3 Flexi® Vector

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pFC17K HaloTag® CMVd3 Flexi® Vector

 Components

  • pFC17K HaloTag® CMVd3 Flexi® Vector

    G132A1 x 20μg
20μg
- G1321 $ 224.00 Add to cart


Available Separately

Product Size Conc. Catalog # *List Price Order QTY Add to Cart

HaloTag® Cloning Starter System

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HaloTag® Cloning Starter System

  Components

  • Wizard® SV Gel and PCR Clean-Up System

    A92801 x 10 preps
  • Flexi® System, Entry/Transfer

    C86411 x 1 each
  • HaloTag® Flexi® Vectors—CMV Deletion Series Sample Pack

    G37801 x 9 × 2μg
  • Carboxy Flexi® Enzyme Blend (SgfI & EcoICRI)

    R19011 x 50μl
1 each
- G6050 $ 329.00 Add to cart

HaloTag® Flexi® Vectors—CMV Deletion Series Sample Pack

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HaloTag® Flexi® Vectors—CMV Deletion Series Sample Pack

  Components

  • pFC17K HaloTag® CMVd3 Flexi® Vector

    G132B1 x 2μg
  • pFC16K HaloTag® CMVd2 Flexi® Vector

    G157B1 x 2μg
  • pFC15K HaloTag® CMVd1 Flexi® Vector

    G160B1 x 2μg
  • pFN21A HaloTag® CMV Flexi® Vector

    G282B1 x 2μg
  • pFN21K HaloTag® CMV Flexi® Vector

    G283B1 x 2μg
  • pFN22K HaloTag® CMVd1 Flexi® Vector

    G285B1 x 2μg
  • pFN23K HaloTag® CMVd2 Flexi® Vector

    G287B1 x 2μg
  • pFN24K HaloTag® CMVd3 Flexi® Vector

    G298B1 x 2μg
  • pFC14K HaloTag® CMV Flexi® Vector

    G966B1 x 2μg
9 × 2μg
- G3780 $ 224.00 Add to cart

You Also May Be Interested In

GenBank®/EMBL Accession Number

pHTC HaloTag® CMV-neo Accession Number JF920305; pHTC HaloTag® CMV-neo Vector sequence text file.
pFC27A HaloTag® CMV-neo Accession Number JN122283; pFC27A HaloTag® CMV-neo Vector sequence text file.
pFC27K HaloTag® CMV-neo Accession Number JN122284; pFC27K HaloTag® CMV-neo Vector sequence text file.
pFC14A HaloTag® CMV Accession Number EU113046; pFC14A HaloTag® CMV Vector sequence text file.
pFC14K HaloTag® CMV Accession Number EU113047; pFC14A HaloTag® CMV Vector sequence text file.
pFC15A HaloTag® CMVd1 Accession Number EU332337; pFC15A HaloTag® CMVd1 Vector sequence text file.
pFC15K HaloTag® CMVd1 Accession Number EU332338; pFC15K HaloTag® CMVd1 Vector sequence text file.
pFC16A HaloTag® CMVd2 Accession Number EU332339; pFC16A HaloTag® CMVd2 Vector sequence text file.
pFC16K HaloTag® CMVd2 Accession Number EU332340; pFC16K HaloTag® CMVd2 Vector sequence text file.
pFC17A HaloTag® CMVd3 Accession Number EU332341; pFC17A HaloTag® CMVd3  Vector sequence text file.
pFC17K HaloTag® CMVd3 Accession Number EU332342; pFC17K HaloTag® CMVd3 Vector sequence text file.

Storage Conditions

Store vectors at –20°C.

For product intended use please see Patents & Disclaimers tab.

pHTC HaloTag® CMV-neo VectorFigure 1. pHTC HaloTag® CMV-neo Vector
pFC27A HaloTag® CMV-neo Flexi® VectorFigure 2. pFC27A HaloTag® CMV-neo Flexi® Vector
pFC27K HaloTag® CMV-neo Flexi® VectorFigure 3. pFC27K HaloTag® CMV-neo Flexi® Vector
pFC14A HaloTag CMV Flexi Vector. Figure 4. pFC14A HaloTag® CMV Flexi® Vector.
pFC14K HaloTag CMV Flexi Vector.Figure 5. pFC14K HaloTag® CMV Flexi® Vector.
pFC15A HaloTag CMVd1 Flexi Vector.Figure 6. pFC15A HaloTag® CMVd1 Flexi® Vector.
pFC15K HaloTag CMVd1 Flexi Vector. Figure 7. pFC15K HaloTag® CMVd1 Flexi® Vector.
pFC16A HaloTag CMVd2 Flexi Vector.Figure 8. pFC16A HaloTag® CMVd2 Flexi® Vector.
pFC16K HaloTag CMVd2 Flexi Vector. Figure 9. pFC16K HaloTag® CMVd2 Flexi® Vector.
pFC17A HaloTag CMVd3 Flexi Vector. Figure 10. pFC17A HaloTag® CMVd3 Flexi® Vector.
pFC17K HaloTag CMVd3 Flexi Vector.Figure 11. pFC17K HaloTag® CMVd3 Flexi® Vector.
Relative mammalian protein expression levels for HaloTag Flexi Vectors.Table 1. Relative Mammalian Protein Expression Levels for HaloTag® Flexi® Vectors.

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Use Restrictions

G7711, G8421, G8431, G9651, G9661, G1611, G1601, G1591, G1571, G1551, G1321, G6050, G3780 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

G7711, G8421, G8431, G9651, G9661, G1611, G1601, G1591, G1571, G1551, G1321, G6050, G3780 BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the HaloTag® gene. Researchers may however clone heterologous DNA sequences at either or both ends of said HaloTag® gene so as to create fused gene sequences provided that the coding sequence of the resulting HaloTag® gene has no more than four (4) deoxynucleotides missing at the affected terminus when compared to the intact HaloTag® gene sequence. In addition, researchers must do one of the following in conjunction with use of the product: (1) use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties, or (2) contact Promega to obtain a license if Promega HaloTag® ligands are not to be used. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

G7711, G8421, G8431, G9651, G9661, G1611, G1601, G1591, G1571, G1551, G1321, G6050, G3780 U.S. Pat. Nos. 7,425,436 and 7,935,803 and other patents pending.

G8421, G8431, G9651, G9661, G1611, G1601, G1591, G1571, G1551, G1321, G6050, G3780 European Pat. No. 1685247 and other patents pending.

G6050, G3780 Patent Pending.

G6050 U.S. Pat. No. 5,391,487.

G7711, G8421, G8431, G9651, G9661, G1611, G1601, G1591, G1571, G1551, G1321, G6050, G3780 For research use only. Persons wishing to use this product or its derivatives in other fields of use, including without limitation, commercial sale, diagnostics or therapeutics, should contact Promega Corporation for licensing information.

G6050 Licensed under U.S. Pat. No. 5,945,288.

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