Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, RT-PCR, first-strand cDNA synthesis.
Purified Avian Myeloblastosis Virus particles.
200mM potassium phosphate (pH 7.2 at 4°C), 0.2% Triton® X-100, 2mM DTT and 50% gycerol.
Store at –20°C.
One unit is defined as the amount of enzyme required to catalyze the transfer of 1nmol of dTTP into acid-insoluble form in 10 minutes at 37°C. The reaction conditions are: 50mM Tris-HCl (pH 8.3 at 25°C), 40mM KCl, 8.75mM MgCl2, 10mM DTT, 0.1mg/ml acetylated BSA, 1mM radiolabeled dTTP and 0.25mM poly(A):oligo(dT).
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