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Promega Corporation

GoTaq® 2-Step RT-qPCR System
for Dye-Based DetectionPCR Satisfaction Guaranteed

Performing precise, accurate RT-qPCR analysis can be difficult when the target copy number is low or when PCR inhibitors are present. The GoTaq® 2-Step RT-qPCR System is a reagent system for quantitative analysis of RNA using a 2-step RT-qPCR protocol. The BRYT Green® Fluorescent Dye and optimized buffer formulations allow robust, reliable cDNA synthesis of a full range of rare and abundant transcripts, even in the presence of inhibitors, using the GoScript™ Reverse Transcription System and quantification using the GoTaq® qPCR Master Mix. The system is compatible with all real-time PCR instrumen...

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  • Prices valid for US customers only
Product Size Conc. Catalog # *List Price QTY Add to Cart

GoTaq® 2-Step RT-qPCR System

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GoTaq® 2-Step RT-qPCR System

 Components

  • MgCl2

    A351B1 x 750μl
  • GoScript™ 5X Reaction Buffer

    A500B1 x 300μl
  • GoScript™ Reverse Transcriptase

    A501B1 x 50μl
  • GoTaq® qPCR Master Mix, 2X

    A600A5 x 1ml
  • Oligo(dT)15 Primer

    C110B1 x 50μg
  • PCR Nucleotide Mix

    C114G1 x 200μl
  • Random Primers

    C118B1 x 50μg
  • CXR Reference Dye

    C541A1 x 100μl
  • Recombinant RNasin® Ribonuclease Inhibitor

    N251A1 x 2,500u
  • Nuclease-Free Water

    P119E2 x 13ml
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


50 × 20µl RT reactions + 200 × 50µl qPCR reactions
- A6010 $ 479.00 Add to cart


Available Separately

Product Size Conc. Catalog # *List Price QTY Add to Cart

CXR Reference Dye

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CXR Reference Dye

  Components

  • CXR Reference Dye

    C541A1 x 100μl
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


100μl
- C5411 $ 22.00 Add to cart

Storage Conditions

Upon arrival, store all components at –30 to –10°C, protected from light. For immediate use, components may be stored at 2–8°C, protected from light, for up to 3 months.

For product intended use please see Patents & Disclaimers tab.

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