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Promega Corporation

GoTaq® 2-Step RT-qPCR System
for Dye-Based DetectionPCR Satisfaction Guaranteed

Performing precise, accurate RT-qPCR analysis can be difficult when the target copy number is low or when PCR inhibitors are present. The GoTaq® 2-Step RT-qPCR System is a reagent system for quantitative analysis of RNA using a 2-step RT-qPCR protocol. The BRYT Green® Fluorescent Dye and optimized buffer formulations allow robust, reliable cDNA synthesis of a full range of rare and abundant transcripts, even in the presence of inhibitors, using the GoScript™ Reverse Transcription System and quantification using the GoTaq® qPCR Master Mix. The system is compatible with all real-time PCR instrumen...

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Product Size Conc. Catalog # *List Price Order QTY Add to Cart

GoTaq® 2-Step RT-qPCR System

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GoTaq® 2-Step RT-qPCR System

 Components

  • MgCl2

    A351B1 x 750μl
  • GoScript™ 5X Reaction Buffer

    A500B1 x 300μl
  • GoScript™ Reverse Transcriptase

    A501B1 x 50μl
  • GoTaq® qPCR Master Mix, 2X

    A600A5 x 1ml
  • Oligo(dT)15 Primer

    C110B1 x 50μg
  • PCR Nucleotide Mix

    C114G1 x 200μl
  • Random Primers

    C118B1 x 50μg
  • CXR Reference Dye

    C541A1 x 100μl
  • Recombinant RNasin® Ribonuclease Inhibitor

    N251A1 x 2,500u
  • Nuclease-Free Water

    P119E2 x 13ml
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  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


50 × 20µl RT reactions + 200 × 50µl qPCR reactions
- A6010 $ 479.00 Add to cart


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PRODUCT SIZE CONC CATALOG # *List Price ORDER QTY. Add to Cart

CXR Reference Dye

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CXR Reference Dye

 Components

  • CXR Reference Dye

    C541A1 x 100μl
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


100μl
- C5411 $ 22.00 Add to cart

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Storage Conditions

Upon arrival, store all components at –30 to –10°C, protected from light. For immediate use, components may be stored at 2–8°C, protected from light, for up to 3 months.

For product intended use please see Patents & Disclaimers tab.

9017TAFigure 1. Quantification of an extremely high abundant reference RNA of variable input target over a 9-order dynamic range.

cDNA synthesized in reactions containing input human reference total RNA varied over a 9-log range. Undiluted 10μl samples were quantified in replicate, specific to 5.8S cDNA. GoTaq® 2-Step RT-qPCR System quantifies cDNA representative of 100ng–1fg of input total RNA. Insets: a) standard curve using the various template amounts in each replicate qPCR, 100ng–1fg. Slope = –3.43; R2 = 0.999. b) dissociation curve shows specificity of GoTaq® qPCR reactions and negative NTC.

cDNA synthesized in reactions containing input human reference total RNA varied over a 9-log range. Undiluted 10μl samples were quantified in replicate, specific to 5.8S cDNA. GoTaq® 2-Step RT-qPCR System quantifies cDNA representative of 100ng–1fg of input total RNA. Insets: a) standard curve using the various template amounts in each replicate qPCR, 100ng–1fg. Slope = –3.43; R2 = 0.999. b) dissociation curve shows specificity of GoTaq® qPCR reactions and negative NTC.

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Use Restrictions

A6010, C5411 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

A6010 U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

A6010 NOTICE TO PURCHASER: LIMITED LICENSE
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser’s activities for a fee or other commercial consideration, is conveyed expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404 USA.

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