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The RiboMAX™ Large Scale RNA Production Systems consistently produce 2–5mg/ml of RNA in a 1ml reaction, about 10- to 20-fold more RNA than is produced with the standard Riboprobe® System transcription reaction. The RiboMAX™ System reactions differ from those of the Riboprobe® Systems in three primary ways: a HEPES (pH 7.5) buffer is used rather than a Tris-HCl (pH 7.9) buffer; rNTP and magnesium concentrations are elevated at levels appropriate for either SP6 or T7 RNA polymerase; and inorganic pyrophosphatase is included in the reaction.
RNAs synthesized with the RiboMAX™ System perf...
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RNAs synthesized with the RiboMAX™ System perform better for in vitro translation in rabbit reticulocyte translation systems than RNA synthesized by standard methods. The reduction of components inhibitory to translation may be advantageous for other applications requiring biologically active RNA. Because the RiboMAX™ Systems produce large quantities of RNA, these systems are not recommended for the generation of high-specific-activity RNA probes.
Note: Use of the RiboMAX™ System for production of capped transcripts requires separate purchase of the Ribo m7G Cap Analog (Cat. P1711).
For more information, see the Protocols & Applications Guide.
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RiboMAX™ Large Scale RNA Production System-SP6
SP6 Transcription Buffer, 5X
SP6 Linear Control DNA
Sodium Acetate, 3.0M (pH 5.2)
Enzyme Mix, SP6 RNA Polymerase
RQ1 RNase-Free DNase
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This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.
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RiboMAX™ Large Scale RNA Production System-T7
Enzyme Mix, T7 RNA Polymerase
T7 Transcription Buffer, 5X
T7 Linear Control DNA
Store at –20°C.
For product intended use please see Patents & Disclaimers tab.
P1280, P1300 For Research Use Only. Not for Use in Diagnostic Procedures.
P1280, P1300 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.
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