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Promega Corporation

pGEM®-T Vector Systems

The pGEM®-T Vector Systems are convenient systems to clone PCR products. The pGEM®-T Vector is prepared by cutting the pGEM®-5Zf(+) Vector with EcoRV and adding a 3´ terminal thymidine to both ends. These single 3´-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmid by preventing recircularization of the vector and providing a compatible overhang for ligation of PCR products generated by thermostable polymerases that add a single deoxyadenosine, in a template-independent fashion, to the 3´-ends of amplified fragments.

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  • *Prices valid for US customers only
Product Size Conc. Catalog # *List Price QTY Add to Cart

pGEM®-T Vector System I

20 reactions
- A3600 $ 133.00 Add to cart

pGEM®-T Vector System II

20 reactions
- A3610 $ 243.00 Add to cart

GenBank®/EMBL Accession Number

The pGEM®-T Vector is derived from the pGEM®-5Zf(+) Vector (GenBank® Accession No. X65308). The pGEM®-T Vector was created by linearizing the pGEM®-5Zf(+) Vector with EcoRV at base 51 and adding a T to both 3´-ends. The position of the T is indicated by * in the pGEM®-T Vector Sequence (.txt).

Storage Conditions

Store competent cells at –70°C; store all other components at –20°C.

For product intended use please see Patents & Disclaimers tab.

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