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Promega Corporation

pGEM®-T Vector Systems

The pGEM®-T Vector Systems are convenient systems to clone PCR products. The pGEM®-T Vector is prepared by cutting the pGEM®-5Zf(+) Vector with EcoRV and adding a 3´ terminal thymidine to both ends. These single 3´-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmid by preventing recircularization of the vector and providing a compatible overhang for ligation of PCR products generated by thermostable polymerases that add a single deoxyadenosine, in a template-independent fashion, to the 3´-ends of amplified fragments.

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pGEM®-T Vector System I

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pGEM®-T Vector System I

 Components

  • pGEM®-T Vector

    A362A1 x 1.2μg
  • Control Insert DNA

    A363A1 x 12μl
  • 2X Rapid Ligation Buffer

    C671A1 x 200μl
  • T4 DNA Ligase

    M180A1 x 100u
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  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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20 reactions
- A3600 $ 134.00 Add to cart

pGEM®-T Vector System II

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pGEM®-T Vector System II

 Components

  • pGEM®-T Vector

    A362A1 x 1.2μg
  • Control Insert DNA

    A363A1 x 12μl
  • 2X Rapid Ligation Buffer

    C671A1 x 200μl
  • JM109 Competent Cells, High Efficiency

    L200A6 x 200μl
  • T4 DNA Ligase

    M180A1 x 100u
20 reactions
- A3610 $ 245.00 Add to cart


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GenBank®/EMBL Accession Number

The pGEM®-T Vector is derived from the pGEM®-5Zf(+) Vector (GenBank® Accession No. X65308). The pGEM®-T Vector was created by linearizing the pGEM®-5Zf(+) Vector with EcoRV at base 51 and adding a T to both 3´-ends. The position of the T is indicated by * in the pGEM®-T Vector Sequence (.txt).

Storage Conditions

Store competent cells at –70°C; store all other components at –20°C.

For product intended use please see Patents & Disclaimers tab.

pGEM-T Vector.Figure 1. pGEM®-T Vector.

Use Restrictions

A3600, A3610 For Research Use Only. Not for Use in Diagnostic Procedures.

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