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Use the pGEM®-T Vectors for general PCR cloning and the pTargeT™ Vector when you need to express the insert in mammalian cells.
General PCR Cloning: pGEM®-T Vector Systems
Rapid and reliable system has no need for post-PCR modification. Cloning is complete in one hour at room temperature and easily releases the insert from the vector.
Direct Mammalian Expression: pTargeT™ Vector
Easily express cloned PCR products in mammalian cells using the powerful CMV promoter. Offers increased expression due to the SV40 late poly(A) sequence and origin of replication.
The pGEM®-T Vector System is a convenient system for the cloning of PCR products, designed to improve the efficiency of ligation of the PCR product into the plasmid.
This chapter of the Protocols and Applications Guide provides a background on basic cloning with a focus on cloning PCR fragments.