Cloning Tools and Competent Cells

Single Step KRX Competent Cells

Efficient transformation and tightly controlled protein expression. Ideal for rapidly generating new clones for recombinant protein expression.

Single-Use Pro 5-alpha Competent Cells

High-efficiency competent cells (109cfu/µg), ideal for the blue/white screening of recombinant clones. Pro 5-alpha can be used for the efficient transformation of unmethylated DNA from PCR, cDNA and many other sources.

pGEM® 3Zf+/- Vectors

Multi-purpose, blue/white screening vector with many unique restriction sites. Use as a standard cloning vector, for in vitro transcription, or for production of circular ssDNA.

T4 DNA Ligase

Blue/White cloning qualified. Use to join two strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides in either sticky- or blunt-end configuration.

Featured Resources

BioMath Calculators
Unit 6: Basics of Molecular Cloning (Blue/White Selection)