The Flexi® Cloning System enables high-fidelity transfer of protein-coding regions between vectors. Many vector options allow you to easily move between applications.
Single Step KRX Competent Cells
Efficient transformation and tightly controlled protein expression. Ideal for rapidly generating new clones for recombinant protein expression.
JM109 Competent Cells
High efficiency competent cells, ideal for the blue/white screening of recombinant clones, resulting from subcloning or the generation of cDNA libraries.
pGEM® 3Zf+/- Vectors
Multi-purpose, blue/white screening vector with many unique restriction sites. Use as a standard cloning vector, for in vitro transcription, or for production of circular ssDNA.
T4 DNA Ligase
Blue/White cloning qualified. Use to join two strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides in either sticky- or blunt-end configuration.
The easy way to calculate melting temperature, molarity, dilution, DNA and protein concentration.
This teaching unit covers the basics of cloning and blue/white selection. Includes math-intensive lab