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Western Blue® Stabilized Substrate for Alkaline Phosphatase is a stable, ready-to-use substrate for Western blots and immunoscreening. It is a mixture of 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) and nitro blue tetrazolium (NBT) in a proprietary stabilizing buffer. Western Blue® Substrate should be used directly and without dilution. This liquid substrate deposits a permanent dark purple stain on membrane sites bearing alkaline phosphatase. Western Blue® Substrate is as sensitive as other reagents based on the BCIP/NBT formulation.
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Western Blue® Stabilized Substrate for Alkaline Phosphatase
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This product is available through the Promega Helix onsite stocking program in a room temperature cabinet. The program offers numerous convenient solutions to meet your lab's needs. The Helix Cabinet is available in two sizes: 7.9 cubic ft. or 25 cubic ft.
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Store at room temperature, 22–25°C.
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The blot was performed on a serial dilution of lysate; each lane contains an indicated amount of β-actin (ng). Primary antibody (monoclonal) used at 1:5,000, Anti-Mouse IgG, AP Conjugate secondary antibody (Cat.# S3721) used at 1:2,500; blot imaged with Western® Blue Stabilized Substrate for Alkaline Phosphatase (Cat.# S3841).
S3841 For Research Use Only. Not for Use in Diagnostic Procedures.
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