Deoxyuridine Triphosphate (dUTP)
Prevent Carryover from Previous PCR Amplifications
- Highly pure (≥99% triphosphate)
- Supplied at 100mM in water for ease-of-use
- Incorporate dUTP into PCR products and treat with uracil-N-glycosylase to inhibit subsequent amplifications
- Manufactured under cGMP for consistent, lot-to-lot performance
- Intended for Laboratory Use (GPR) in the United States. Outside of the United States, this product is intended for research use only unless otherwise stated
Catalog Number:
Size
Catalog Number: U1191
High-quality deoxynucleotide triphosphates (dNTPs) are critical for the success of many key procedures such as cDNA synthesis, sequencing, cloning and genotyping. Promega dNTPs have greater than 99% triphosphate content and are provided at a concentration of 100mM in water at pH 7.5.
dUTP (2´-Deoxyuridine, 5´-Triphosphate) can be used in place of dTTP in PCR and RT-PCR protocols to prevent carryover from previous amplifications. The substitution of dUTP for dTTP in PCR results in uracil-containing PCR products that are suitable for most standard applications. The enzyme uracil-N-glycosylase (UNG, also referred to as UDG) can be added to a PCR premix to excise uracil from any contaminating PCR product, thereby preventing false positives. Each lot of dUTP is tested to ensure specific DNA amplification and the absence of nuclease activity.
- Longo, M.C. et al. (1990) Gene 93, 125–8.
- Erlich, H.A. et al. (1991) Science 252, 1643–51.
Protocols
No protocols available
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación | Concentración |
|---|---|---|---|
|
dUTP |
U119A | 1 × 400μl | 100mM |
SDS
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Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Condiciones de Almacenaje
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