M-MLV Reverse Transcriptase
RNA-Dependent DNA Polymerase for cDNA Synthesis
- Enzyme supplied at a concentration of 200u/μl
Catalog Number:
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Catalog Number: M1701
Catalog Number: M1705
Catalog Number: M5313
RNA-Dependent DNA Polymerase for cDNA Synthesis
Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb). The enzyme is a product of the pol gene of M-MLV and consists of a single subunit with a molecular weight of 71kDa. The RNase H activity of M-MLV RT is weaker than that of the commonly used Avian Myeloblastosis Virus (AMV) reverse transcriptase.
Advantages- Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 375mM KCl, 15mM MgCl2, 50mM DTT.
- May Be Heat-Inactivated: M-MLV RT is inactivated by heating at 70°C for 10 minutes.
- Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/
Protocols
Complete Protocol
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación |
|---|---|---|
|
M-MLV Reverse Transcriptase |
M170A | 1 × 10,000u |
|
M-MLV RT 5X Buffer |
M531A | 1 × 1ml |
SDS
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Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación |
|---|---|---|
|
M-MLV Reverse Transcriptase |
M170B | 1 × 50,000u |
|
M-MLV RT 5X Buffer |
M531A | 2 × 1ml |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación |
|---|---|---|
|
M-MLV RT 5X Buffer |
M531A | 2 × 1ml |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Condiciones de Almacenaje
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