AMV Reverse Transcriptase
Efficient Reverse Transcription of Templates with High Secondary Structure
- DNA polymerization using template DNA, RNA or RNA:DNA hybrids
- Requires a primer as well as magnesium or manganese
- Stable at higher reaction temperatures (37–58°C)
- Supplied at a concentration of 10u/μl (Cat.# M5101, M5108) or 20–25u/µl (Cat.# M9004)
- Intended for Laboratory Use (GPR) in the United States. Outside of the United States, this product is intended for research use only unless otherwise stated
Catalog Number:
Size
Catalog Number: M5101
Catalog Number: M5108
Catalog Number: M9004
Reverse Transcriptase with Stability at Higher Temperatures
Avian Myeloblastosis Virus Reverse Transcriptase (AMV RT) catalyzes DNA polymerization using template DNA, RNA or RNA:DNA hybrids. It requires a primer (DNA primers are more efficient than RNA primers) as well as Mg2+ or Mn2+. The enzyme possesses an intrinsic RNase H activity. Both nonionic detergents and sulfhydryl compounds stabilize the enzyme activity in vitro.
Features- High Concentration: Cat.# M9004 contains 600 units of AMV RT at 20–25u/μl.
- 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 250mM KCl, 50mM MgCl2, 2.5mM spermidine, 50mM DTT.
- Temperature Stability: AMV RT is the preferred reverse transcriptase for templates with high secondary structure due to its stability at higher reaction temperatures (37–58°C).
- First- and second-strand synthesis of cDNA.
- Primer extensions and RNA sequencing.
- RT-PCR. Up to 10μl of an RT reaction containing AMV RT and the supplied AMV RT Reaction Buffer can be added to a 50μl PCR amplification reaction that uses Taq DNA polymerase. If GoTaq® DNA Polymerase or PCR Master Mix are used, up to 25μl of an RT reaction can be added per 50μl PCR.
- Kacian, D.L. (1977) Meth. Virol. 6, 143.
- Mierendorf, R.C. and Pfeffer, D. (1987) Meth. Enzymol. 152, 563–6.
Protocols
Complete Protocol
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación |
|---|---|---|
|
AMV Reverse Transcriptase |
M510A | 1 × 300u |
| AMV RT 5X Buffer | M515A | 1 × 500μl |
SDS
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Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación |
|---|---|---|
|
AMV Reverse Transcriptase |
M510F | 1 × 1,000u |
| AMV RT 5X Buffer | M515A | 3 × 500μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
| AMV RT 5X Buffer | M515A | 1 × 500μl | |
|
AMV Reverse Transcriptase (HC) |
M900A | 1 × 600u | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Condiciones de Almacenaje
Resources
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