CellTiter-Glo® 2.0 cell viability assay can be multiplexed with other assays, reducing cell culture costs and labor. Multiplexing assays in the same sample well allows complementary measures of cell health to correct for errors in culturing, plating or assay chemistry interferences.
In the example shown here, CellTox™ Green cytotoxicity assay reagent was added to bortezemib-treated K562 cells after 48 hours of exposure. Fluorescence associated with cytotoxicity was measured. The CellTiter-Glo® 2.0 assay reagent was added and luminescence measured as an indicator of cell proliferation.