Mammalian Expression Vectors

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Bacterial Expression

Cloning

Lambda

Mutagenesis

Mammalian Expression

pGEM^®-luc,
ΦX174 & pSP

Reporter

RNA Interference

T Vectors

Complete Vector List

Technical Resources

CheckMate™/Flexi^® HaloTag™ pHT2 pACT pAdVAntage™ pALTER^®-MAX pBIND	pCAT^®3 pCI pCMVTNT™ pG5luc phRG	phRL pSI pTARGET™ pTNT™ Regulated Mammalian Expression Discontinued

Checkmate™/Flexi^® Vectors
Enlarge map	pFN10A (ACT) Flexi^® (Cat.# C9360) Protocol: CheckMate™/Flexi^® Vector Mammalian Two-Hybrid System, TM283 Sequence and reference points GenBank^® Acc.#: DQ487211

Enlarge map	pFN11A (BIND) Flexi^® (Cat.# C9360) Protocol: CheckMate™/Flexi^® Vector Mammalian Two-Hybrid System, TM283 Sequence and reference points GenBank^® Acc.#: DQ487212

Enlarge map	*pGL4.31[luc2P/GAL4UAS/Hygro]* (Cat.# C9351) Sequence and reference points Protocol: CheckMate™/Flexi^® Vector Mammalian Two-Hybrid System, TM283 GenBank^® Acc.#: DQ487213

HaloTag™ pHT2 Vector
Enlarge map	HaloTag™ pHT2 (Cat.# G8241) Sequence and reference points Protocol: HaloTag™ Interchangeable Labeling Technology, TM260 GenBank^® Acc.#: AY773970

pACT Vectors
Enlarge map	pACT (Cat.# E2440) Component of the CheckMate™ Mammalian Two-Hybrid System. Sequence and reference points Multiple cloning region Protocol: CheckMate™ Mammalian Two-Hybrid System, TM049 GenBank^® Acc.#: AF264723 MCR Restriction Enzymes: BamH I, Sal I, Acc I, Mlu I, EcoR V, Xba I, BstZ I, Not I, Kpn I, Acc65 I

Enlarge map	pACT-MyoD Control (Cat.# E2440) Component of the CheckMate™ Mammalian Two-Hybrid System. Protocol: CheckMate™ Mammalian Two-Hybrid System, TM049 GenBank^® Acc.#: None

pAdVAntage™ Vector
Enlarge map	pAdVAntage™ (Cat.# E1711) Lambda replacement vector derived from lambda 1059. Sequence and reference points Protocol: pAdVAntage™ Vector Technical Bulletin, TB207 GenBank^® Acc.#: U47294

pALTER^®-MAX Vector
Enlarge map	pALTER^®-MAX (Cat.# Q5761) Designed for use in mutagenesis and bacterial expression. Sequence and reference points Multiple cloning region Protocol: Altered Sites^® II Mammalian Mutagenesis System, TM041 GenBank^® Acc.#: None MCR restriction enzymes: Xho I, Acc I, EcoR I, Mlu I, Kpn I, Xba I, Sal I, Sma I, Not I

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pBIND Vectors
Enlarge map	pBIND (Cat.# E2440) This vector is a component of the CheckMate Two-Hybrid System. Sequence and reference points Multiple cloning region Protocol: CheckMate^® Mammalian Two-Hybrid System, TM049 GenBank^® Acc.#: AF264722 MCR Restriction Enzymes: BamH I, Sal I, Acc I, Mlu I, Xba I, EcoR V, BstZ I, Not I, Kpn I, Acc65 I

Enlarge map	pBIND-Id Control (Cat.# E2440) This vector is a component of the CheckMate Two-Hybrid System. Protocol: CheckMate^® Mammalian Two-Hybrid System, TM049 GenBank^® Acc.#: None

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pCAT^®3 Vectors
Enlarge map	pCAT^®3-Basic (Cat.# E1871) Lacks eukaryotic promoter and enhancer sequences, allowing maximum flexibility in cloning putative mammalian regulatory sequences. Sequence and reference points Multiple cloning region Protocol: pCAT^®3 Reporter Vectors, TM036 GenBank^® Acc.#: U57024 MCR Restriction Enzymes: Kpn I, Sac I, Mlu I, Nhe I, Sma I, Xho I, Bgl II, Sal I, BamH I

Enlarge map	pCAT^®3-Control (Cat.# E1851) Contains SV40 promoter and enhancer sequences resulting in strong expression of CAT in many mammalian cells. Sequence and reference points Multiple cloning region Protocol: pCAT^®3 Reporter Vectors, TM036 GenBank^® Acc.#: U57025 MCR restriction enzymes: Kpn I, Sac I, Mlu I, Nhe I, Xma I, Xho I, Bgl II, Sal I, BamH I

Enlarge map	pCAT^®3-Enhancer (Cat.# E1881) SV40 enhancer located downstream of the CAT gene and poly(A) signal aids in verification of functional promoter elements. Sequence and reference points Multiple cloning region Protocol: pCAT^®3 Reporter Vectors, TM036 GenBank^® Acc.#: U57026 MCR restriction enzymes: Hind III, SphI, Pst I, Sal I, Acc I, Hinc II, Xba I, Ava I, BamH I, Sma I, Kpn I, Sac I, EcoR I

Enlarge map	pCAT^®3-Promoter (Cat.# E1861) Contains an SV40 promoter upstream of the intron and CAT gene. Sequence and reference points Multiple cloning region Protocol: pCAT^®3 Reporter Vectors, TM036 GenBank^® Acc.#: U57027 MCR restriction enzymes: Kpn I, Sac I, Mlu I, Sal I, Nhe I, Sma I, Xho I, Bgl II, BamH I

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pCI Vectors
Enlarge map	pCI (Cat.# E1731) Designed to promote constitutive expression of cloned DNA inserts in mammalian cells. Sequence and reference points Multiple cloning region Protocol: pCI and pSI Mammalian Expression Vectors, TB206 GenBank^® Acc.#: U47119 MCR restriction enzymes: Nhe I, Xho I, EcoR I, Mlu I, Kpn I, Xba I, Sal I, Acc I, Sma I, BstZ I, Not I

Enlarge map	pCI-neo (Cat.# E1841) Mammalian expression vector. Sequence and reference points MCS sequence GenBank^® Acc.#: U47120 MCR restriction enzymes: Nhe I, Xho I, EcoR I, Mlu I, Xba I, Sal I, Acc I, Sma I, Not I

pCMTNT™ Vector
Enlarge map	pCMVTNT™ (Cat.# L5620) Designed for in vitro and in vivo expression of cloned genes. Sequence and reference points Multiple cloning region Protocol: pCMVTNT™ Vector, TB305 GenBank^® Acc.#: AF477200 MCR restriction enzymes: Xho I, EcoR I, Mlu I, Kpn I, Xba I, Sal I, Acc I, Sma I, BstZ I, Not I

pG5luc Vector
Enlarge map	pG5luc (Cat.# E2440) This vector is a component of the CheckMate™ Mammalian Two-Hybrid System. Sequence and reference points Protocol: CheckMate™ Mammalian Two-Hybrid System, TM049 GenBank^® Acc.#: AF264724

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phRG Vectors
Enlarge map	phRG-B (Cat.# E6281) Mammalian expression, reporter vector. Sequence and reference points Multiple cloning region Protocol: Synthetic Renilla Luciferase Reporter Vectors, TM237 GenBank^® Acc.#: AF362550 MCR restriction enzymes: Kpn I, Sac I, Mlu I, Nhe I, Sma I, Xho I, Bgl II, Hind III

Enlarge map	phRG-TK (Cat.# E6291) Mammalian expression, reporter vector. Sequence and reference points Protocol: Synthetic Renilla Luciferase Reporter Vectors, TM237 GenBank^® Acc.#: AF362551

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phRL Vectors
Enlarge map	phRL-CMV (Cat.# E6271) Mammalian expression, reporter vector. Sequence and reference points Protocol: Synthetic Renilla Luciferase Reporter Vectors, TM237 GenBank^® Acc.#: AF362549

Enlarge map	phRL-null (Cat.# E6231) Mammalian expression, reporter vector. Sequence and reference points Multiple cloning region Protocol: Synthetic Renilla Luciferase Reporter Vectors, TM237 GenBank^® Acc.#: AF362546 MCR restriction enzymes: Bgl II, Xho I, Sac I, Hind III, EcoICR I, Nde I, Sph I, Spe I, Nar I, Sal I, Mlu I, EcoR I, Sma I, Pst I

Enlarge map	phRL-SV40 (Cat.# E6261) Mammalian expression, reporter vector. Sequence and reference points Protocol: Synthetic Renilla Luciferase Reporter Vectors, TM237 GenBank^® Acc.#: AF362548

Enlarge map	phRL-TK (Cat.# E6241) Mammalian expression, reporter vector. Sequence and reference points Protocol: Synthetic Renilla Luciferase Reporter Vectors, TM237 GenBank^® Acc.#: AF362545

Enlarge map	phRL-TK(Int–) (Cat.# E6251) Mammalian expression, reporter vector. Sequence and reference points Protocol: Synthetic Renilla Luciferase Reporter Vectors, TM237 GenBank^® Acc.#: AF362547

pSI Vector
Enlarge map	pSI (Cat.# E1721) Designed to promote constitutive expression of cloned DNA inserts in mammalian cells. Sequence and reference points Multiple cloning region Protocol: pCI and pSI Mammalian Expression Vectors, TB206 GenBank^® Acc.#: U47121 MCR restriction enzymes: Nhe I, Xho I, EcoR I, Mlu I, Xba I, Sal I, Acc I, Sma I, BstZ I, Not I

pTARGET™ Vector
Enlarge map	pTARGET™ (Cat.# A1410) Mammalian expression and PCR cloning vector. Sequence and reference points Multiple cloning region Protocol: pTARGET™ Mammalian Expression System, TM044 GenBank^® Acc.#: None MCR restriction enzymes: EcoR I, BamH I, Nhe I, Xho I, Mlu I, Sma I, Kpn I, Sal I, Acc I, Not I

pTNT™ Vector
Enlarge map	pTNT™ (Cat.# L5610) In vitro and protein expression. Sequence and reference points Multiple cloning region Protocol: pTNT™ Vector, TB304 GenBank^® Acc.#: AF479322 MCR restriction enzymes: Xho I, EcoR I, Mlu I, Kpn I, Xba I, Sal I, Acc I, Sma I, BstZ I, Not I

Regulated Mammalian Expression Vectors
Enlarge map	pF12A RM Flexi^® (Cat.# C9431) Protocol: Regulated Mammalian Expression System, TM289 Sequence and reference points GenBank^® Acc.#: EF030520

Enlarge map	pF12K RM Flexi^® (Cat.# C9441) Protocol: Regulated Mammalian Expression System, TM289 Sequence and reference points GenBank^® Acc.#: EF030521

Enlarge map	pReg neo (Cat.# C9421) Protocol: Regulated Mammalian Expression System, TM289 Sequence and reference points GenBank^® Acc.#: EF030522

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Discontinued
Enlarge map	pCAT^®-Basic (discontinued) GenBank^® Acc.#: X65322

Enlarge map	pCAT^®-Control (discontinued) GenBank^® Acc.#: X65321

Enlarge map	pCAT^®-Enhancer (discontinued) GenBank^® Acc.#: X65319

Enlarge map	pCAT^®-Promoter (discontinued) GenBank^® Acc.#: X65320

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