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Vectors
Bacterial Expression
Cloning
Lambda
Mutagenesis
Mammalian Expression
pGEM®-luc, ΦX174 & pSP
Reporter
RNA Interference
T Vectors
Complete Vector List
Technical Resources
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Technical Resources
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Mammalian Expression Vectors
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CheckMate™/Flexi®
HaloTag™ pHT2
pACT
pAdVAntage™
pALTER®-MAX
pBIND |
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pCAT®3
pCI
pCMVTNT™
pG5luc
phRG |
phRL
pSI
pTARGET™
pTNT™
Regulated Mammalian Expression
Discontinued
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Checkmate™/Flexi® Vectors |
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pFN10A (ACT) Flexi® (Cat.#
C9360)
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pFN11A (BIND) Flexi® (Cat.#
C9360)
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pGL4.31[luc2P/GAL4UAS/Hygro]
(Cat.#
C9351)
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HaloTag™ pHT2 Vector
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HaloTag™ pHT2 (Cat.#
G8241)
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pACT Vectors
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pACT (Cat.#
E2440)
Component of the CheckMate™ Mammalian Two-Hybrid System.
- Sequence and reference points
- Multiple cloning region
- Protocol: CheckMate™
Mammalian Two-Hybrid System, TM049
- GenBank® Acc.#: AF264723
- MCR Restriction Enzymes:
BamH
I, Sal
I, Acc
I, Mlu
I, EcoR
V, Xba
I, BstZ
I, Not
I, Kpn
I, Acc65
I
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pACT-MyoD Control (Cat.#
E2440)
Component of the CheckMate™ Mammalian Two-Hybrid System.
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pAdVAntage™ Vector
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pAdVAntage™ (Cat.#
E1711)
Lambda replacement vector derived from lambda 1059.
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pALTER®-MAX Vector
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pALTER®-MAX (Cat.#
Q5761)
Designed for use in mutagenesis and bacterial expression.
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pBIND Vectors
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pBIND (Cat.#
E2440)
This vector is a component of the CheckMate Two-Hybrid System.
- Sequence and
reference points
- Multiple cloning region
- Protocol: CheckMate®
Mammalian Two-Hybrid System, TM049
- GenBank® Acc.#: AF264722
- MCR Restriction Enzymes:
BamH
I, Sal
I, Acc
I, Mlu
I, Xba
I,
EcoR
V, BstZ
I, Not
I, Kpn
I, Acc65
I
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pBIND-Id Control (Cat.#
E2440)
This vector is a component of the CheckMate Two-Hybrid System.
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pCAT®3 Vectors
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pCAT®3-Basic (Cat.#
E1871)
Lacks eukaryotic promoter and enhancer sequences, allowing
maximum flexibility in cloning putative mammalian regulatory
sequences.
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pCAT®3-Control (Cat.#
E1851)
Contains SV40 promoter and enhancer sequences resulting in strong
expression of CAT in many mammalian cells.
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pCAT®3-Enhancer (Cat.#
E1881)
SV40 enhancer located downstream of the CAT gene and poly(A) signal
aids in verification of functional promoter elements.
- Sequence and reference points
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Multiple cloning region
- Protocol: pCAT®3
Reporter Vectors, TM036
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GenBank® Acc.#: U57026
- MCR restriction enzymes:
Hind
III, SphI,
Pst
I, Sal
I, Acc
I,
Hinc
II, Xba
I, Ava
I, BamH
I, Sma
I, Kpn
I, Sac
I,
EcoR
I
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pCAT®3-Promoter (Cat.#
E1861)
Contains an SV40 promoter upstream of the intron and CAT gene.
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pCI Vectors
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pCI (Cat.#
E1731)
Designed to promote constitutive expression of cloned DNA inserts
in mammalian cells.
- Sequence and reference points
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Multiple cloning region
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Protocol: pCI and pSI
Mammalian Expression Vectors, TB206
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GenBank® Acc.#: U47119
- MCR restriction enzymes: Nhe
I,
Xho
I, EcoR
I, Mlu
I, Kpn
I, Xba
I, Sal
I, Acc
I, Sma
I, BstZ
I, Not
I
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pCI-neo (Cat.#
E1841)
Mammalian expression vector.
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pCMTNT™ Vector
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pCMVTNT™ (Cat.#
L5620)
Designed for in vitro and in vivo expression of cloned genes.
- Sequence and reference points
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Multiple cloning region
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Protocol: pCMVTNT™
Vector, TB305
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GenBank® Acc.#: AF477200
- MCR restriction enzymes:
Xho
I,
EcoR
I, Mlu
I, Kpn
I, Xba
I, Sal
I, Acc
I, Sma
I, BstZ
I, Not
I
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pG5luc Vector
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pG5luc (Cat.#
E2440)
This vector is a component of the CheckMate™ Mammalian Two-Hybrid
System.
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phRG Vectors
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phRG-B (Cat.#
E6281)
Mammalian expression, reporter vector.
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phRG-TK (Cat.#
E6291)
Mammalian expression, reporter vector.
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phRL Vectors
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phRL-CMV (Cat.#
E6271)
Mammalian expression, reporter vector.
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phRL-null (Cat.#
E6231)
Mammalian expression, reporter vector.
- Sequence and reference points
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Multiple cloning region
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Protocol: Synthetic
Renilla Luciferase Reporter Vectors, TM237
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GenBank® Acc.#: AF362546
- MCR restriction enzymes:
Bgl
II, Xho
I, Sac
I, Hind
III,
EcoICR
I, Nde
I, Sph
I, Spe
I,
Nar
I, Sal
I, Mlu
I, EcoR
I,
Sma
I, Pst
I
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phRL-SV40 (Cat.#
E6261)
Mammalian expression, reporter vector.
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phRL-TK (Cat.#
E6241)
Mammalian expression, reporter vector.
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phRL-TK(Int–) (Cat.#
E6251)
Mammalian expression, reporter vector.
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pSI Vector
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pSI (Cat.#
E1721)
Designed to promote constitutive expression of cloned DNA inserts
in mammalian cells.
- Sequence and reference points
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Multiple cloning region
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Protocol: pCI and
pSI Mammalian Expression Vectors, TB206
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GenBank® Acc.#: U47121
- MCR restriction enzymes:
Nhe
I, Xho
I, EcoR
I, Mlu
I,
Xba
I, Sal
I, Acc
I, Sma
I,
BstZ
I,
Not
I
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pTARGET™ Vector
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pTARGET™ (Cat.#
A1410)
Mammalian expression and PCR cloning vector.
- Sequence and reference points
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Multiple cloning region
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Protocol: pTARGET™ Mammalian Expression
System, TM044
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GenBank® Acc.#: None
- MCR restriction enzymes:
EcoR
I, BamH
I, Nhe
I, Xho
I,
Mlu
I, Sma
I, Kpn
I, Sal
I,
Acc
I, Not
I
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pTNT™ Vector
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pTNT™ (Cat.#
L5610)
In vitro and protein expression.
- Sequence and reference points
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Multiple cloning region
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Protocol: pTNT™
Vector, TB304
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GenBank® Acc.#: AF479322
- MCR restriction enzymes:
Xho
I, EcoR
I, Mlu
I, Kpn
I, Xba
I,
Sal
I, Acc
I, Sma
I, BstZ
I, Not
I
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Regulated Mammalian Expression Vectors |
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pF12A RM Flexi® (Cat.#
C9431)
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pF12K RM Flexi® (Cat.#
C9441)
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pReg neo (Cat.#
C9421)
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Discontinued
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pCAT®-Basic (discontinued)
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pCAT®-Control (discontinued)
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pCAT®-Enhancer (discontinued)
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pCAT®-Promoter (discontinued)
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