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pBR322 (Cat.# D1511) 

pBR322 DNA digests are useful as molecular weight size markers in nucleic acid gel analysis.

• Sequence and reference points

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pCAT^®3-Basic (Cat.# E1871) 

Lacks eukaryotic  promoter and enhancer sequences, allowing maximum flexibility in cloning putative mammalian regulatory sequences.

• Sequence and reference points
• Multiple cloning region
• Protocol: pCAT^®3 Reporter Vectors, TM036
• GenBank^® Acc.#: U57024
• MCR Restriction Enzymes: Kpn I, Sac I, Mlu I,  
  Nhe I, Sma I, Xho I, Bgl II, Sal I, BamH I

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pCAT^®3-Control (Cat.# E1851)

Contains SV40 promoter and enhancer sequences resulting in strong expression of CAT in many mammalian cells.

• Sequence and reference points
• Multiple cloning region
• Protocol: pCAT^®3 Reporter Vectors, TM036
• GenBank^® Acc.#: U57025
• MCR restriction enzymes: Kpn I, Sac I, Mlu I,  
  Nhe I, Xma I,  Xho I, Bgl II, Sal I, BamH I

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pCAT^®3-Enhancer (Cat.# E1881)

SV40 enhancer located downstream of the CAT gene and poly(A) signal aids in verification of functional promoter elements.

• Sequence and reference points
• Multiple cloning region
• Protocol: pCAT^®3 Reporter Vectors, TM036
• GenBank^® Acc.#: U57026
• MCR restriction enzymes: Hind III, SphI, Pst I,
  Sal I, Acc I,  Hinc II, Xba I, Ava I, BamH I, 
  Sma I, Kpn I, Sac I,  EcoR I

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pCAT^®3-Promoter  (Cat.# E1861)

Contains an SV40 promoter upstream of the intron and CAT gene.

• Sequence and reference points
• Multiple cloning region
• Protocol: pCAT^®3 Reporter Vectors, TM036
• GenBank^® Acc.#: U57027
• MCR restriction enzymes: Kpn I, Sac I, Mlu I,  
  Sal I, Nhe I, Sma I, Xho I, Bgl II, BamH I

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: AY753576

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: AY753577

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: AY949043

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: AY949044

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: AY753580

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: AY753581

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: DQ487155

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: DQ487156

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: DQ871024

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: DQ133906

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: DQ133907

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: EU113046

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: EU113047

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• Sequence and reference points
• GenBank^® Acc.#: EU545996

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• Sequence and reference points
• GenBank^® Acc.#: EU545997

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: AY753578

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: AY753579

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: DQ132629

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: DQ132630

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• Sequence and reference points
• GenBank^® Acc.#: EU545992

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• Sequence and reference points
• GenBank^® Acc.#: EU545994

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• Sequence and reference points
• GenBank^® Acc.#: EU545994

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• Sequence and reference points
• GenBank^® Acc.#: EU545995

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pGEM^®-3Z (Cat.# P2151)

• Sequence and reference points
• Multiple cloning region
• Protocol: pGEM^®-3Z Vector, TB033
• GenBank^® Acc.#: X65304
• MCR restriction enzymes: Hind III, SphI, Pst I, 
  Sal I, Acc I, Hinc II, Xba I, Ava I, BamH I, Sma I, 
  Kpn I, Sac I, EcoR I

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pGEM^®-3Zf(+)  (Cat.# P2271)

• Sequence and reference points
• Multiple cloning region
• Protocol: pGEM^®-3Zf(+) Vector, TB086
• GenBank^® Acc.#: X65306
• MCR restriction enzymes: EcoR I, Sac I, Kpn I,  
  Ava I, Sma I, Xba I, BamH I, Sal I, Acc I, Hinc II,
  Pst I, Sph I, Hind III

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pGEM^®-3Zf(-)  (Cat.# P2261)

This vector is designed for cloning PCR products.

• Sequence and reference points
• Multiple cloning region
• Protocol: pGEM^®-3Zf(-) Vector, TB045
• GenBank^® Acc.#: X65307
• MCR restriction enzymes: EcoR I, Sac I, Kpn I,  
  Ava I, Sma I, Xba I, BamH I, Sal I, Acc I, Hinc II,
  Pst I, Sph I, Hind III

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pGEM^®-4Z (Cat.# P2161)

Cloning, in vitro transcription.

• Sequence and reference points
• Multiple cloning region 
• Protocol: pGEM^®-4Z Vector, TB036
• GenBank^® Acc.#: X65305
• MCR restriction enzymes: EcoR I, Sac I, Kpn I, 
  Ava I, Xma I, Sma I, BamH I, Xba I, Sal I, Acc I, Hinc II, Pst I, Sph I, Hind III

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pGEM^®-5Zf(+)  (Cat.# P2241)

Cloning, in vitro transcription.

• Sequence and reference points
• Multiple cloning region 
• Protocol: pGEM^®-5Zf(+) Vector, TB047
• GenBank^® Acc.#: X65308
• MCR restriction enzymes: Apa I, Aat II, Sph I,
  Nco I, Sac II,Spe I, EcoR V, Not I, Pst I, Sal I, 
  Nde I, Sac I, BstX I, Nsi I

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pGEM^®-5Zf(-)  (Cat.# P2351)

Cloning, in vitro transcription.

• Sequence and reference points
• Multiple cloning region 
• Protocol: pGEM^®-5Zf(-) Vector, TB068
• GenBank^® Acc.#: X65309
• MCR restriction enzymes: Apa I, Aat II, Sph I,  
  Nco I, Sac II,Spe I, EcoR V, Not I, Pst I, Sal I, 
  Nde I, Sac I, BstX I, Nsi I

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pGEM^®-7Zf(+) (Cat.# P2251)

Cloning, in vitro transcription.

• Sequence and reference points
• Multiple cloning region
• Protocol: pGEM^®-7Zf(+) Vector, TB048
• GenBank^® Acc.#: X65310
• MCR restriction enzymes: Apa I, Aat II, Sph I,  
  Xba I, Xho I, EcoR I, Kpn I, Sma I, Csp45 I, Cla I, Sac I, Hind III, BamH I, BstX I, Nsi I

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pGEM^®-7Zf(-) (Cat.# P2371)

Cloning, in vitro transcription.

• Sequence and reference points
• Multiple cloning region
• Protocol: pGEM^®-7Zf(-) Vector, TB069
• GenBank^® Acc.#: X65311
• MCR restriction enzymes: Apa I, Aat II, Sph I, 
  Xba I, Xho I, EcoR I, Kpn I, Sma I, Csp45 I, Cla I, Sac I, Hind III, BamH I, BstX I, Nsi I

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pGEM^®-9Zf(-) (Cat.# P2391)

Cloning, in vitro transcription, ssDNA synthesis.

• Sequence and reference points
• Multiple cloning region 
• Protocol: pGEM^®-9Zf(-) Vector, TB070
• GenBank^® Acc.#: X65312
• MCR restriction enzymes: Nsi I, Spe I, Hind III, Xba I, EcoR I, Sal I, Sac I, Tth111 I, Not I, Sfi I

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pGEM^®-11Zf(+) (Cat.# P2411)

Cloning, in vitro transcription, ssDNA synthesis.

• Sequence and reference points
• Multiple cloning region
• Protocol: pGEM^®-11Zf(+) Vector, TB075
• GenBank^® Acc.#: X65313
• MCR restriction enzymes: Sfi I, Sac I, Eco R I,  
  Sal I, Xho I, Apa I, BamH I, Xba I, Not I, Sph I, Nsi I, Hind III

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pGEM^®-11Zf(-) (Cat.# P2421)

Cloning, in vitro transcription, ssDNA synthesis.

• Sequence and reference points
• Multiple cloning region 
• Protocol: pGEM^®-11Zf(-) Vector, TB074
• GenBank^® Acc.#: X65314
• MCR restriction enzymes: Sfi I, Sac I, EcoR I, 
  Sal I, Xho I, Apa I, BamH I, Xba I, Not I, Sph I, 
  Nsi I, Hind III

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pGEM^®-13Zf(+) (Cat.# P2541)

Cloning, in vitro transcription, ssDNA synthesis.

• Sequence and reference points
• Multiple cloning region 
• Protocol: pGEM^®-13Zf(+) Vector, TB073
• GenBank^® Acc.#: X65315
• MCR restriction enzymes: Not I, Sfi I, Hind III

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pGL2-Basic (Cat.# E1641)

Lacks eukaryotic promoter and enhancer sequences, allowing maximum flexibility in cloning putative regulatory sequences.

• Sequence and reference points
• Multiple cloning region
• Protocol: pGL2 Luciferase Reporter Vectors, TM003
• GenBank^® Acc.#: X65323
• MCR restriction enzymes: Sma I, Kpn I, Sac I,  
  Mlu I,  Nhe I, Xho I, Bgl II, Hind III

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pGL2-Control (Cat.# E1611)

Contains SV40 promoter and enhancer sequences resulting in strong luc expression in many mammalian cells.

• Sequence and reference points
• Multiple cloning region
• Protocol: pGL2 Luciferase Reporter Vectors, TM003
• GenBank^® Acc.#: X65324
• MCR restriction enzymes:  Sma I, Kpn I, Sac I, Mlu I, Nhe I, Xho I, Bgl II

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pGL2-Enhancer (Cat.# E1621)

Contains an SV40 enhancer derived from the SV40 early promoter, located downstream of the luc gene after the poly(A) signal.

• Sequence and reference points
• Multiple cloning region
• Protocol: pGL2 Luciferase Reporter Vectors, TM003
• GenBank^® Acc.#: X65325
• MCR restriction enzymes: Sma I, Kpn I, Sac I,  
  Mlu I, Nhe I, Xho I, Bgl II, Hind III

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pGL2-Promoter (Cat.# E1631)

Contains the SV40 promoter upstream of the luc gene.

• Sequence and reference points
• Multiple cloning region
• Protocol: pGL2 Luciferase Reporter Vectors, TM003
• GenBank^® Acc.#: X65326
• MCR restriction enzymes: Sma I, Kpn I, Sac I,  
  Mlu I, Nhe I, Xho I, Bgl II

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pG5luc (Cat.# E2440)

This vector is a component of the CheckMate™ Mammalian Two-Hybrid System.

• Sequence and reference points
• Protocol: CheckMate™ Mammalian Two-Hybrid System, TM049
• GenBank^® Acc.#: AF264724

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pGL3-Basic (Cat.# E1751)

Lacks eukaryotic promoter and enhancer sequences, allowing maximum flexibility in cloning putative regulatory sequences.

• Sequence and reference points
• Multiple cloning region
• Protocol: pGL3 Luciferase Reporter Vectors, TM033
• GenBank^® Acc.#: U47295
• MCR restriction enzymes: Kpn I, Sac I, Mlu I,  
  Nhe I, Sma I, Xho I, Bgl II, Hind III

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pGL3-Control (Cat.# E1741)

Contains SV40 promoter and enhancer sequences resulting in strong expression of luc+ in many mammalian cell types.

• Sequence and reference points
• Multiple cloning region
• Protocol:pGL3 Luciferase Reporter Vectors, TM033
• GenBank^® Acc.#: U47296
• MCR restriction enzymes: Kpn I, Sac I, Mlu I, 
  Nhe I, Sma I, Xho I, Bgl II

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pGL3-Enhancer (Cat.# E1771)

Contains an SV40 enhancer located downstream of luc+ and the poly(A) signal.

• Sequence and reference points
• Multiple cloning region
• Protocol: pGL3 Luciferase Reporter Vectors, TM033
• GenBank^® Acc.#: U47297
• MCR restriction enzymes: Kpn I, Sac I, Mlu I,  
  Nhe I, Sma I, Xho I, Bgl II, Hind III

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pGL3-Promoter (Cat.# E1761)

Contains an SV40 promoter upstream of the luc+ gene.

• Sequence and reference points

• Multiple cloning region
• Protocol: pGL3 Luciferase Reporter Vectors, TM033
• GenBank^® Acc.#: U47298
• MCR restriction enzymes: Kpn I, Sac I, Mlu I,  
  Nhe I, Sma I, Xho I, Bgl II

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pSP64 Poly(A) (Cat.# P1241)

Cloning, in vitro transcription.

• Sequence and reference points
• Multiple cloning region
• Protocol: pSP64 Poly(A) Vector Sequence and Map, TB052
• GenBank^® Acc.#: X65328
• MCR restriction enzymes: Hind III, Pst I, Sal I, 
  Acc I, Hinc II, Xba I, Ava I, BamH I, Sma I, Sac I, EcoR I

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pSP72 (Cat.# P2191)

Cloning, in vitro transcription.

• Sequence and reference points

• Multiple cloning region
• Protocol: pSP72 Vector Sequence and Map, TB040
• GenBank^® Acc.#: X65332
• MCR restriction enzymes: Xho I, Pvu I, Hind III, 
  Sph I, Pst I, Sal I, Acc I, BamH I, Sma I, Kpn I, 
  Sac I, EcoR I, Cla I, EcoR V, Bgl II, Xba I

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pSP73 (Cat.# P2221)

Cloning, in vitro transcription.

• Sequence and reference points
• Mulitple cloning region
• Protocol: pSP73 Vector Sequence and Map, TB041
• GenBank^® Acc.#: X65333
• MCR restriction enzymes: Bgl II, EcoR V, Cla I, EcoR I, Sac I, Kpn I, Sma I, Xba I, BamH I, Sal I, Acc I, Pst I, Sph I, Hind III, Pvu II, Xho I

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EMBL3 Genomic Cloning (discontinued) 

Lambda replacement vector derived from lambda 1059.

• For sequence see GenBank^® 
  Acc.#: U02453 (right arm)
  and  U02425 (left arm)
• MCR restriction enzymes: Sal I, BamH I, EcoR I

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Lambda replacement vector derived from lambda 1059.

• Identical to EMBL3 except that multiple cloning region is in the reverse orientation. EMBL4 has same stuffer fragment as EMBL3.
• GenBank^® Acc.#: None
• MCR Restriction Enzymes: Sal I, BamH I, EcoR I

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Multifunctional replacement type genomic cloning vector that accepts DNA fragments of 9-23kb.

• Protocol: None
• For sequence see GenBank^® 
  Acc.#: U02427  (right arm)
  and U02426 (left arm) 
• MCR restriction enzymes: Sfi I, Sac I, Xho I, 
  BamH I, Avr II, EcoR I, Xba I

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LambdaGem-12 Genomic Cloning (discontinued) 

Lambda replacement vector derived from lambda 1059.

• Protocol: None
• LambdaGem-12 arms are identical to EMBL3 arms (above) except that they have different multiple cloning regions containing T7 and SP6 RNA polymerase promoters and recognition sites listed below. LambdaGem-12 has a different stuffer fragment than EMBL3.
• MCR restriction enzymes: Sfi I, Sac I, Not I, BamH I, EcoR I, Xho I

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Lambda gt10 (discontinued) 

cDNA cloning vector.

• Protocol: None
• The polylinker region is GenBank^® Acc.#: U02447

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Lambda gt11 (discontinued) 

cDNA cloning vector.

• Protocol: None
• The polylinker region is X16973
  The lac region is X04125

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Lambda gt11 Sfi-Not (discontinued) 

cDNA cloning vector.

• Protocol: None
• GenBank^® Acc.#: None

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pCAT^® Basic (discontinued)

• GenBank^® Acc.#: X65322

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pCAT^® Control (discontinued)

• GenBank^® Acc.#: X65321

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pCAT^® Enhancer (discontinued)

• GenBank^® Acc.#: X65319

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pCAT^® Promoter (discontinued)

• GenBank^® Acc.#: X65320

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pGEM^®-1 (discontinued)

• Sequence and reference points
• GenBank^® Acc.#: X65300
• MCR restriction enzymes: Hind III, Pst I, Sal I, Acc I, Sma I, Hinc II, Xba I, BamH I, Ava I, Sac I, EcoR I

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pGEM^®-2 (discontinued)

• Sequence and reference points
• GenBank^® Acc.#: X65301
• MCR restriction enzymes: EcoR I, Sac I, Ava I, Sma I, BamH I, Xba I, Sal I, Acc I, Hinc II, Pst I, Hind III

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pGEM^®-3 (discontinued)

• Sequence and reference points
• GenBank^® Acc.#: X65302
• MCR restriction enzymes: Hind III, SphI, Pst I, Sal I, Acc I, Hinc II, Xba I, Ava I, BamH I, Sma I, Kpn I, Sac I, EcoR I

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pGEM^®-4 Vector (discontinued)

• Sequence and reference points
• GenBank^® Acc.#: X65307
• MCR restriction enzymes: EcoR I, Sac I, Kpn I, Ava I, Sma I, Xba I, BamH I, Sal I, Acc I, Hinc II, Pst I, Sph I, Hind III

pGEM^®-15Zf(-) (discontinued)

Cloning.

• Sequence and reference points

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pSP64 (discontinued)

Cloning, in vitro transcription.

• Sequence and reference points
• GenBank^® Acc.#: X65327
• MCR restriction enzymes: Hind III, Pst I, Sal I, Acc I, Hinc II,Xba I, Ava I, BamH I, Sma I, Sac I, EcoR I

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pSP65 (discontinued)

Cloning, in vitro transcription.

• Sequence and reference points
• GenBank^® Acc.#: X65329
• MCR restriction enzymes: EcoR I, Sac I, Ava I, Sma I, Xba I, BamH I, Sal I, Acc I, Hinc II, Pst I, Hind III

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pSP70 (discontinued)

Cloning, in vitro transcription.

• Sequence and reference points
• GenBank^® Acc.#: X65330
• MCR restriction enzymes: Xho I, Pvu I, Hind III, EcoR I, Cla I, EcoR V, Bgl II