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pBR322 (Cat.# D1511) 

pBR322 DNA digests are useful as molecular weight size markers in nucleic acid gel analysis.

• Sequence and reference points

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pCAT^®3-Basic (Cat.# E1871) 

Lacks eukaryotic  promoter and enhancer sequences, allowing maximum flexibility in cloning putative mammalian regulatory sequences.

• Sequence and reference points
• Multiple cloning region
• Protocol: pCAT^®3 Reporter Vectors, TM036
• GenBank^® Acc.#: U57024
• MCR Restriction Enzymes: Kpn I, Sac I, Mlu I,  
  Nhe I, Sma I, Xho I, Bgl II, Sal I, BamH I

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pCAT^®3-Control (Cat.# E1851)

Contains SV40 promoter and enhancer sequences resulting in strong expression of CAT in many mammalian cells.

• Sequence and reference points
• Multiple cloning region
• Protocol: pCAT^®3 Reporter Vectors, TM036
• GenBank^® Acc.#: U57025
• MCR restriction enzymes: Kpn I, Sac I, Mlu I,  
  Nhe I, Xma I,  Xho I, Bgl II, Sal I, BamH I

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pCAT^®3-Enhancer (Cat.# E1881)

SV40 enhancer located downstream of the CAT gene and poly(A) signal aids in verification of functional promoter elements.

• Sequence and reference points
• Multiple cloning region
• Protocol: pCAT^®3 Reporter Vectors, TM036
• GenBank^® Acc.#: U57026
• MCR restriction enzymes: Hind III, SphI, Pst I,
  Sal I, Acc I,  Hinc II, Xba I, Ava I, BamH I, 
  Sma I, Kpn I, Sac I,  EcoR I

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pCAT^®3-Promoter  (Cat.# E1861)

Contains an SV40 promoter upstream of the intron and CAT gene.

• Sequence and reference points
• Multiple cloning region
• Protocol: pCAT^®3 Reporter Vectors, TM036
• GenBank^® Acc.#: U57027
• MCR restriction enzymes: Kpn I, Sac I, Mlu I,  
  Sal I, Nhe I, Sma I, Xho I, Bgl II, BamH I

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: AY753576

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: AY753577

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: AY949043

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: AY949044

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: AY753580

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: AY753581

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: DQ487155

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: DQ487156

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: DQ871024

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• Protocol: TNT^® T7 Insect Cell Extract System, TM305
• Sequence and reference points
• GenBank^® Acc.#: EU754721

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• Protocol: TNT^® T7 Insect Cell Extract System, TM305
• Sequence and reference points
• GenBank^® Acc.#: EU754722

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: DQ133906

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: DQ133907

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: EU113046

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: EU113047

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• Sequence and reference points
• GenBank^® Acc.#: EU332337

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• Sequence and reference points
• GenBank^® Acc.#: EU332338

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• Sequence and reference points
• GenBank^® Acc.#: EU332339

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• Sequence and reference points
• GenBank^® Acc.#: EU332340

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• Sequence and reference points
• GenBank^® Acc.#: EU545996

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• Sequence and reference points
• GenBank^® Acc.#: EU332342

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• Sequence and reference points
• GenBank^® Acc.#: EU545996

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• Sequence and reference points
• GenBank^® Acc.#: EU545997

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: AY753578

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: AY753579

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: DQ132629

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• Protocol: Flexi^® Vector Systems, TM254
• Sequence and reference points
• GenBank^® Acc.#: DQ132630

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• Sequence and reference points
• GenBank^® Acc.#: EU545992

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• Sequence and reference points
• GenBank^® Acc.#: EU545994

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• Sequence and reference points
• GenBank^® Acc.#: EU545994

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• Sequence and reference points
• GenBank^® Acc.#: EU545995

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• Sequence and reference points
• GenBank^® Acc.#: EU621374

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• Sequence and reference points
• GenBank^® Acc.#: EU621375

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• Sequence and reference points
• GenBank^® Acc.#: EU621376

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• Sequence and reference points
• GenBank^® Acc.#: EU621377

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• Sequence and reference points
• GenBank^® Acc.#: EU621378

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• Sequence and reference points
• GenBank^® Acc.#: EU621379

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• Sequence and reference points
• GenBank^® Acc.#: EU621380

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• Sequence and reference points
• GenBank^® Acc.#: EU621381

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This vector is a component of the CheckMate™ Mammalian Two-Hybrid System.

• Sequence and reference points
• Protocol: CheckMate™ Mammalian Two-Hybrid System, TM049
• GenBank^® Acc.#: AF264724

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pGEM^®-3Z (Cat.# P2151)

• Sequence and reference points
• Multiple cloning region
• Protocol: pGEM^®-3Z Vector, TB033
• GenBank^® Acc.#: X65304
• MCR restriction enzymes: Hind III, SphI, Pst I, 
  Sal I, Acc I, Hinc II, Xba I, Ava I, BamH I, Sma I, 
  Kpn I, Sac I, EcoR I

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pGEM^®-3Zf(+)  (Cat.# P2271)

• Sequence and reference points
• Multiple cloning region
• Protocol: pGEM^®-3Zf(+) Vector, TB086
• GenBank^® Acc.#: X65306
• MCR restriction enzymes: EcoR I, Sac I, Kpn I,  
  Ava I, Sma I, Xba I, BamH I, Sal I, Acc I, Hinc II,
  Pst I, Sph I, Hind III

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pGEM^®-3Zf(-)  (Cat.# P2261)

This vector is designed for cloning PCR products.

• Sequence and reference points
• Multiple cloning region
• Protocol: pGEM^®-3Zf(-) Vector, TB045
• GenBank^® Acc.#: X65307
• MCR restriction enzymes: EcoR I, Sac I, Kpn I,  
  Ava I, Sma I, Xba I, BamH I, Sal I, Acc I, Hinc II,
  Pst I, Sph I, Hind III

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pGEM^®-4Z (Cat.# P2161)

Cloning, in vitro transcription.

• Sequence and reference points
• Multiple cloning region 
• Protocol: pGEM^®-4Z Vector, TB036
• GenBank^® Acc.#: X65305
• MCR restriction enzymes: EcoR I, Sac I, Kpn I, 
  Ava I, Xma I, Sma I, BamH I, Xba I, Sal I, Acc I, Hinc II, Pst I, Sph I, Hind III

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pGEM^®-5Zf(+)  (Cat.# P2241)

Cloning, in vitro transcription.

• Sequence and reference points
• Multiple cloning region 
• Protocol: pGEM^®-5Zf(+) Vector, TB047
• GenBank^® Acc.#: X65308
• MCR restriction enzymes: Apa I, Aat II, Sph I,
  Nco I, Sac II,Spe I, EcoR V, Not I, Pst I, Sal I, 
  Nde I, Sac I, BstX I, Nsi I

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pGEM^®-5Zf(-)  (Cat.# P2351)

Cloning, in vitro transcription.

• Sequence and reference points
• Multiple cloning region 
• Protocol: pGEM^®-5Zf(-) Vector, TB068
• GenBank^® Acc.#: X65309
• MCR restriction enzymes: Apa I, Aat II, Sph I,  
  Nco I, Sac II,Spe I, EcoR V, Not I, Pst I, Sal I, 
  Nde I, Sac I, BstX I, Nsi I

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pGEM^®-7Zf(+) (Cat.# P2251)

Cloning, in vitro transcription.

• Sequence and reference points
• Multiple cloning region
• Protocol: pGEM^®-7Zf(+) Vector, TB048
• GenBank^® Acc.#: X65310
• MCR restriction enzymes: Apa I, Aat II, Sph I,  
  Xba I, Xho I, EcoR I, Kpn I, Sma I, Csp45 I, Cla I, Sac I, Hind III, BamH I, BstX I, Nsi I

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pGEM^®-7Zf(-) (Cat.# P2371)

Cloning, in vitro transcription.

• Sequence and reference points
• Multiple cloning region
• Protocol: pGEM^®-7Zf(-) Vector, TB069
• GenBank^® Acc.#: X65311
• MCR restriction enzymes: Apa I, Aat II, Sph I, 
  Xba I, Xho I, EcoR I, Kpn I, Sma I, Csp45 I, Cla I, Sac I, Hind III, BamH I, BstX I, Nsi I

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pGEM^®-9Zf(-) (Cat.# P2391)

Cloning, in vitro transcription, ssDNA synthesis.

• Sequence and reference points
• Multiple cloning region 
• Protocol: pGEM^®-9Zf(-) Vector, TB070
• GenBank^® Acc.#: X65312
• MCR restriction enzymes: Nsi I, Spe I, Hind III, Xba I, EcoR I, Sal I, Sac I, Tth111 I, Not I, Sfi I

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pGEM^®-11Zf(+) (Cat.# P2411)

Cloning, in vitro transcription, ssDNA synthesis.

• Sequence and reference points
• Multiple cloning region
• Protocol: pGEM^®-11Zf(+) Vector, TB075
• GenBank^® Acc.#: X65313
• MCR restriction enzymes: Sfi I, Sac I, Eco R I,  
  Sal I, Xho I, Apa I, BamH I, Xba I, Not I, Sph I, Nsi I, Hind III

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pGEM^®-11Zf(-) (Cat.# P2421)

Cloning, in vitro transcription, ssDNA synthesis.

• Sequence and reference points
• Multiple cloning region 
• Protocol: pGEM^®-11Zf(-) Vector, TB074
• GenBank^® Acc.#: X65314
• MCR restriction enzymes: Sfi I, Sac I, EcoR I, 
  Sal I, Xho I, Apa I, BamH I, Xba I, Not I, Sph I, 
  Nsi I, Hind III

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pGEM^®-13Zf(+) (Cat.# P2541)

Cloning, in vitro transcription, ssDNA synthesis.

• Sequence and reference points
• Multiple cloning region 
• Protocol: pGEM^®-13Zf(+) Vector, TB073
• GenBank^® Acc.#: X65315
• MCR restriction enzymes: Not I, Sfi I, Hind III

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pGL2-Basic (Cat.# E1641)

Lacks eukaryotic promoter and enhancer sequences, allowing maximum flexibility in cloning putative regulatory sequences.

• Sequence and reference points
• Multiple cloning region
• Protocol: pGL2 Luciferase Reporter Vectors, TM003
• GenBank^® Acc.#: X65323
• MCR restriction enzymes: Sma I, Kpn I, Sac I,  
  Mlu I,  Nhe I, Xho I, Bgl II, Hind III

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pGL2-Control (Cat.# E1611)

Contains SV40 promoter and enhancer sequences resulting in strong luc expression in many mammalian cells.

• Sequence and reference points
• Multiple cloning region
• Protocol: pGL2 Luciferase Reporter Vectors, TM003
• GenBank^® Acc.#: X65324
• MCR restriction enzymes:  Sma I, Kpn I, Sac I, Mlu I, Nhe I, Xho I, Bgl II

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pGL2-Enhancer (Cat.# E1621)

Contains an SV40 enhancer derived from the SV40 early promoter, located downstream of the luc gene after the poly(A) signal.

• Sequence and reference points
• Multiple cloning region
• Protocol: pGL2 Luciferase Reporter Vectors, TM003
• GenBank^® Acc.#: X65325
• MCR restriction enzymes: Sma I, Kpn I, Sac I,  
  Mlu I, Nhe I, Xho I, Bgl II, Hind III

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pGL2-Promoter (Cat.# E1631)

Contains the SV40 promoter upstream of the luc gene.

• Sequence and reference points
• Multiple cloning region
• Protocol: pGL2 Luciferase Reporter Vectors, TM003
• GenBank^® Acc.#: X65326
• MCR restriction enzymes: Sma I, Kpn I, Sac I,  
  Mlu I, Nhe I, Xho I, Bgl II

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pGL3-Basic (Cat.# E1751)

Lacks eukaryotic promoter and enhancer sequences, allowing maximum flexibility in cloning putative regulatory sequences.

• Sequence and reference points
• Multiple cloning region
• Protocol: pGL3 Luciferase Reporter Vectors, TM033
• GenBank^® Acc.#: U47295
• MCR restriction enzymes: Kpn I, Sac I, Mlu I,  
  Nhe I, Sma I, Xho I, Bgl II, Hind III

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pGL3-Control (Cat.# E1741)

Contains SV40 promoter and enhancer sequences resulting in strong expression of luc+ in many mammalian cell types.

• Sequence and reference points
• Multiple cloning region
• Protocol:pGL3 Luciferase Reporter Vectors, TM033
• GenBank^® Acc.#: U47296
• MCR restriction enzymes: Kpn I, Sac I, Mlu I, 
  Nhe I, Sma I, Xho I, Bgl II

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pGL3-Enhancer (Cat.# E1771)

Contains an SV40 enhancer located downstream of luc+ and the poly(A) signal.

• Sequence and reference points
• Multiple cloning region
• Protocol: pGL3 Luciferase Reporter Vectors, TM033
• GenBank^® Acc.#: U47297
• MCR restriction enzymes: Kpn I, Sac I, Mlu I,  
  Nhe I, Sma I, Xho I, Bgl II, Hind III

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pGL3-Promoter (Cat.# E1761)

Contains an SV40 promoter upstream of the luc+ gene.

• Sequence and reference points

• Multiple cloning region
• Protocol: pGL3 Luciferase Reporter Vectors, TM033
• GenBank^® Acc.#: U47298
• MCR restriction enzymes: Kpn I, Sac I, Mlu I,  
  Nhe I, Sma I, Xho I, Bgl II

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pSP64 Poly(A) (Cat.# P1241)

Cloning, in vitro transcription.

• Sequence and reference points
• Multiple cloning region
• Protocol: pSP64 Poly(A) Vector Sequence and Map, TB052
• GenBank^® Acc.#: X65328
• MCR restriction enzymes: Hind III, Pst I, Sal I, 
  Acc I, Hinc II, Xba I, Ava I, BamH I, Sma I, Sac I, EcoR I

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pSP72 (Cat.# P2191)

Cloning, in vitro transcription.

• Sequence and reference points

• Multiple cloning region
• Protocol: pSP72 Vector Sequence and Map, TB040
• GenBank^® Acc.#: X65332
• MCR restriction enzymes: Xho I, Pvu I, Hind III, 
  Sph I, Pst I, Sal I, Acc I, BamH I, Sma I, Kpn I, 
  Sac I, EcoR I, Cla I, EcoR V, Bgl II, Xba I

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pSP73 (Cat.# P2221)

Cloning, in vitro transcription.

• Sequence and reference points
• Mulitple cloning region
• Protocol: pSP73 Vector Sequence and Map, TB041
• GenBank^® Acc.#: X65333
• MCR restriction enzymes: Bgl II, EcoR V, Cla I, EcoR I, Sac I, Kpn I, Sma I, Xba I, BamH I, Sal I, Acc I, Pst I, Sph I, Hind III, Pvu II, Xho I

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