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Vectors
Bacterial Expression
Cloning
Lambda
Mutagenesis
Mammalian Expression
pGEM®-luc,
ΦX174 & pSP
Reporter
RNA Interference
T Vectors
Complete Vector List
Technical Resources
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Technical Resources
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Bacterial Expression Vectors
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PinPoint™
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Discontinued
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PinPoint™ Vectors
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PinPoint™ Xa-1 (Cat.#
V2031)
Bacterial expression.
- Sequence and reference points
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MCS sequence
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Protocol: PinPoint™
Xa Protein Purification System, TM028
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GenBank® Acc.#: U47626
- MCS restriction enzymes: NruI,
PvuI,
HindIII,
BamHI,
Acc65I,
KpnI,
BglII,
EcoRV,
SmaI,
NotI
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Discontinued Bacterial Expression
Vectors
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pALTER®-EX1 (discontinued) Contains a novel polylinker that allows protein expression in
vivo and in vitro.
- Sequence and
reference points
- MCS sequence
- Protocol: Altered
Sites® II in vitro Mutagenesis Systems, TM001
- GenBank® Acc.#: U47102
- MCS Restriction Enzymes:
EcoRI,
HpaI,
NdeI, Nco
I,
StuI,
XbaI,
PstI,
NotI,
SphI,
NsiI, BamHI,
HindIII
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pALTER®-EX2 (discontinued) Contains the same cloning sites and transcription/translation
signals as pALTER®-EX1, but used the p15a origin or
replication.
- Sequence and reference points
- MCS sequence
- Protocol: Altered
Sites® II in vitro Mutagenesis Systems, TM001
- GenBank® Acc.#: U47103
- MCS restriction enzymes:
EcoRI,
HpaI,
NcoI,
StuI,
PstI,
NotI,
SphI,
NsiI,
NdeI,
BamHI,
HindIII
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pET-5a (discontinued)
Bacterial expression.
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pET-5b (discontinued)
Bacterial expression.
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pET-5c (discontinued)
Bacterial expression.
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pET-9a (discontinued)
Bacterial expression.
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pET-9b (discontinued)
Bacterial expression.
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pET-9c (discontinued)
Bacterial expression.
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pGEMEX®-1 (discontinued) Designed for cloning, as templates for in vitro transcription and
for production of ssDNA.
- Sequence and reference points
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MCS sequence
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GenBank® Acc.#: X65317
- MCS restriction enzymes:
HindIII,
NsiI,
SphI,
NotI,
ApaI,
XhoI,
BamHI,
SalI,
EcoRI,
SacI,
SfiI
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pGEMEX®-2 (discontinued) Designed for cloning, as templates for in vitro transcription and
for production of ssDNA.
- Sequence and reference points
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MCS sequence
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GenBank® Acc.#: X65318
- MCS restriction enzymes:
HindIII,
NsiI,
SphI,
NotI,
ApaI,
XhoI,
BamHI,
SalI,
EcoRI,
SacI,
SfiI
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Enlarge
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PinPoint™ Control (discontinued)
Bacterial expression.
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Enlarge
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PinPoint™ Xa Control (discontinued) Bacterial expression.
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Vector
map and sequence are nearly identical to PinPoint™ Xa-1
(above), but contain one
additional adenosine at position 394.
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PinPoint™ Xa-2 (discontinued) Bacterial expression.
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MCS sequence
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Protocol: PinPoint™
Xa Protein Purification System, TM028
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GenBank® Acc.#: U47627
- MCS restriction enzymes: NruI,
PvuI,
HindIII,
BamHI,
Acc65I,
KpnI,
BglII,
EcoRV,
SmaI,
NotI
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Vector
map and sequence are nearly identical to PinPoint™ Xa-1
(above), but contain two additional adenosines at position 394.
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PinPoint™ Xa-3 (discontinued) Bacterial expression.
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MCS sequence
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Protocol: PinPoint™
Xa Protein Purification System, TM028
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GenBank® Acc.#: U47628
- MCS restriction enzymes:
NruI,
PvuI,
HindIII,
BamHI,
Acc65I,
KpnI,
BglII,
EcoRV,
SmaI,
NotI
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