ReliaPrep™ FFPE gDNA Miniprep System Technical Manual

Instructions for Use of Product(s)
A2351, A2352
Literature # TM352

The ReliaPrep™ FFPE gDNA Miniprep System uses incubation conditions optimized to partially reverse cross-linking caused by formalin fixation without the need for an overnight digestion. This system also incorporates a deparaffinization method that does not rely on xylene or other hazardous or volatile solvents.

Summary of Changes
The following changes were made to the 12/15 revision of this document:

  1. Blue Dye is now included in the reagents.
  2. A step was added for preparing the Lysis Buffer (LBA) with Blue Dye.
  3. The lysis step of the methodology now undergoes a 4-hour incubation at 80°C instead of one hour.
  4. An optional overnight storage step was added after the 80°C incubation of the lysis step.

Printed in USA. Revised 12/15.