Nano-Glo® Luciferase Assay System Technical Manual

Instructions for Use of Product(s)
N1110, N1120, N1130, N1150

Literature # TM369

Nano-Glo® Luciferase Assay System provides a simple, single-addition reagent that generates a glow-type signal in the presence of NanoLuc® luciferase. The reagent is prepared by mixing Nano-Glo® Luciferase Assay Substrate and Nano-Glo® Luciferase Assay Buffer and contains an integral lysis buffer to use directly on cells expressing NanoLuc® luciferase or culture medium when the luciferase is secreted.
NanoLuc® (Nluc) luciferase is a small (19.1kDa) engineered luminescent reporter. The enzyme is about
150-fold brighter than either firefly (Photinus pyralis) or Renilla reniformis luciferase, using a novel coelenterazine analog (furimazine) to produce high intensity, glow-type luminescence. The luminescent reaction is ATP-independent and designed to suppress background luminescence for maximal assay sensitivity.

Summary of Changes
The following changes were made to the 1/22 revision of this document:
1. Step 2 in Section 4.B was updated to include examples of the volume of Nano-Glo® Luciferase to use.
2. A disclaimer was added.
3. The cover image was updated.

Revised 1/22.