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Promega Corporation

P450-Glo™ Screening Systems Technical Bulletin

Literature # TB340

The P450-Glo™ Screening Systems provide a complete set of reagents for performing luminescent cytochrome P450 assays. The systems include a membrane preparation containing recombinant human cytochrome P450 enzyme, a luminogenic cytochrome P450 substrate appropriate for the enzyme, an NADPH Regeneration System, reaction buffer, Luciferin Detection Reagent and Luciferin-Free Water. The membranes are prepared from baculovirus-infected insect cells and contain human cytochrome P450 and P450 reductase (and cytochrome b5 for CYP2C9 and CYP3A4). The P450-Glo™ Screening Systems also contain a membrane fraction devoid of cytochrome P450 activity as a negative control. The assays are ideal for testing the effects of drugs and new chemical entities on cytochrome P450 enzyme activities.

Summary of Changes
The following changes were made to the 7/14 revision of this document:
1. The P450-Glo™ CYP2B6 Screening System (Cat.# V9781) and supporting information was added.
2. The document design was updated.

Printed in USA. Revised 7/14.


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