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Promega Corporation

LigaFast™ Rapid DNA Ligation System Product Information

Literature # 9PIM822

The LigaFast™ Rapid DNA Ligation System is designed for the efficient ligation of cohesive-ended DNA inserts into plasmid vectors in just 5 minutes (blunt-ended inserts in as little as 15 minutes). Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation Buffer. The LigaFast™ System is designed to eliminate any further purification prior to transformation of ligated DNA. The specially formulated 2X Rapid Ligation Buffer requires no ATP or Mg2+ addition prior to use.

Printed in USA. Revised 8/13.

 

PDF Icon Complete Protocol PDF (146kb)

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