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Promega Corporation

psiCHECK™-1 and psiCHECK™-2 Vectors

The psiCHECK™-1 and psiCHECK™-2 Vectors are designed to provide a quantitative and rapid approach for initial optimization of RNA interference (RNAi). The vectors enable monitoring of changes in expression of a target gene fused to a reporter gene. In both vectors Renilla luciferase is used as the primary reporter gene, and the gene of interest is cloned into a multiple cloning region located downstream of the Renilla translational stop codon. Initiation of the RNAi process by synthetic siRNAs or in vivo-expressed shRNAs toward a gene of interest results in cleavage and subsequent degradation of the fusion mRNA. M...

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psiCHECK™-1 Vector

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psiCHECK™-1 Vector

 Components

  • psiCHECK™-1 Vector

    C801A1 x 20μg
20μg
- C8011 $ 355.00 Add to cart

psiCHECK™-2 Vector

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psiCHECK™-2 Vector

 Components

  • psiCHECK™-2 Vector

    C802A1 x 20μg
20μg
- C8021 $ 384.00 Add to cart


GenBank®/EMBL Accession Number

psiCHECK™-1 Accession Number AY535006; psiCHECK™-1 Vector sequence text file.
psiCHECK™-2 Accession Numbers AY535007; psiCHECK™-2 Vector sequence text file.

Storage Conditions

Store at –20°C.

For product intended use please see Patents & Disclaimers tab.

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