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Suppress protein expression transiently or permanently using tools designed for producing high efficiency RNAi responses. Produce milligram amounts of double-stranded RNA (dsRNA) suitable for RNAi in both mammalian and nonmammalian systems.
Rapidly screen RNAi by quantifying the Renilla luciferase reporter gene activity. Avoid labor-intensive, time-consuming assays and waiting for phenotypic changes.
Generate milligram amounts of protein-free dsRNA in less than 30 minutes. The dsRNA is suitable for RNAi in both mammalian and nonmammalian systems.
Quantitatively measure microRNA activity with a luciferase reporter. The firefly luciferase (luc2) vector is the primary reporter to monitor mRNA regulation and Renilla luciferase (hRluc-neo) acts as a control reporter for normalization and selection.