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Promega Corporation

MagneGST™ Pull-Down System

The MagneGST™ Pull-Down System is designed for detection of protein interactions between GST-fusion proteins expressed in bacterial lysates and prey proteins expressed in the TnT® Systems. Prey protein synthesized in the TnT® Quick Coupled Transcription/Translation Reaction is captured using bait protein (GST-fusion protein) immobilized on MagneGST™ Particles. Nonspecifically bound proteins are then washed away, and the prey protein is analyzed. Prey proteins can be detected by incorporating radioactively labeled methionine in the TnT® Quick reaction, followed by SDS-PAGE and autoradiography or by incorporating th...

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Product Size Conc. Catalog # *List Price QTY Add to Cart

MagneGST™ Pull-Down System

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MagneGST™ Pull-Down System

 Components

  • Methionine

    L118A1 x 50μl
  • RQ1 RNase-Free DNase

    M610A1 x 1,000u
  • Nuclease-Free Water

    P119A1 x 1,250μl
  • MagneGST™ Cell Lysis Reagent

    V481B1 x 60ml
  • MagneGST™ Glutathione Particles

    V861A1 x 4ml
  • MagneGST™ Binding/Wash Buffer

    V870B2 x 200ml
  • TnT® T7 Quick Master Mix

    V88711 x 8 × 200μl
80 reactions
- V8870 $ 643.00 Add to cart


Storage Conditions

Store the TnT® T7 Quick Master Mix and Methionine at –70°C. Store the RQ1 RNase-Free DNase at –20°C. Store the Nuclease-Free Water, MagneGST™ Glutathione Particles, MagneGST™ Binding/Wash Buffer and Cell Lysis Reagent at 4°C.

For product intended use please see Patents & Disclaimers tab.

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