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Promega Corporation

M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant

Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus (M-MLV RT [H–]), Point Mutant, is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long RNA templates (>5kb). The lack of RNase H activity is beneficial for this application, as RNase H can start to degrade templates when incubation times are long, as they may be when synthesizing long cDNAs. Although many researchers are successful in using M-MLV RT (H+) for analytical and some preparative cDNA applications, reverse transcriptases lacking RNase H activity provide another option to prepare l...

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M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant

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M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant

 Components

  • M-MLV RT, RNase (H–), Point Mutant

    M368A1 x 2,500 units
  • M-MLV RT 5X Buffer

    M531A1 x 1ml
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  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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2,500u
- M3681 $ 40.00 Add to cart

M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant

Close Window

M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant

 Components

  • M-MLV RT, RNase (H–), Point Mutant

    M368B1 x 10,000 units
  • M-MLV RT 5X Buffer

    M531A1 x 1ml
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


10,000u
- M3682 $ 150.00 Add to cart

M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant

Close Window

M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant

 Components

  • M-MLV RT, RNase (H–), Point Mutant

    M368C1 x 50,000 units
  • M-MLV RT 5X Buffer

    M531A5 x 1ml
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


50,000u
- M3683 $ 560.00 Add to cart


QC Tests

Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, specific performance tests.

Source

Recombinant E. coli strain.

Storage Buffer

20mM Tris-HCl (pH 7.5 at 25°C), 200mM NaCl, 0.1mM EDTA, 1mM DTT, 0.01% Nonidet® P-40 and 50% glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is the amount of enzyme required to incorporate 1nmol of deoxynucleotide into acid-precipitable material in 10 minutes at 37°C. The reaction conditions are: 50mM Tris-HCl (pH 8.3 at 25°C), 7mM MgCl2, 40mM KCl, 10mM DTT, 0.1mg/ml BSA, 0.5mM [3H]dTTP, 0.025mM oligo(dT), 0.25mM poly(A) and 0.01% NP-40.

For product intended use please see Patents & Disclaimers tab.

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