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Promega Corporation

T7 RNA Polymerase

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T7 RNA Polymerase is a DNA-dependent RNA polymerase that exhibits extremely high specificity for its cognate promoter sequences. Only T7 DNA or DNA cloned downstream from a T7 promoter can serve as a template for T7 RNA Polymerase-directed RNA synthesis.

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T7 RNA Polymerase

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T7 RNA Polymerase

 Components

  • DTT

    P117B1 x 250μl
  • Transcription Optimized 5X Buffer

    P118B1 x 500μl
  • T7 RNA Polymerase

    P207B1 x 1,000u
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


1,000u
10–20u/μl P2075 $ 72.00 Add to cart

T7 RNA Polymerase

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T7 RNA Polymerase

 Components

  • DTT

    P117C1 x 1.25ml
  • Transcription Optimized 5X Buffer

    P118C1 x 1.25ml
  • T7 RNA Polymerase

    P207E1 x 5,000u
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


5,000u
10–20u/μl P2077 $ 243.00 Add to cart

T7 RNA Polymerase (HC)

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T7 RNA Polymerase (HC)

 Components

  • DTT

    P117B1 x 250μl
  • Transcription Optimized 5X Buffer

    P118B1 x 500μl
  • T7 RNA Polymerase (HC)

    P407A1 x 10,000u
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


10,000u
80u/μl P4074 $ 402.00 Add to cart


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QC Tests

Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, transcription.

Source

Recombinant E. coli strain.

Storage Buffer

20mM potassium phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCI, 0.1% (v/v) Triton® X-100 and 50% (v/v) glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the incorporation of 5nmol of CTP into acid-insoluble product in 60 minutes at 37°C in a total volume of 100μl. The reaction conditions are: 40mM Tris-HCl (pH 7.9 at 25°C), 6mM MgCl2,10mM DTT, 10mM NaCl2, 2mM spermidine, 0.05% Tween® 20, 0.5mM each of ATP, GTP, CTP, and UTP, 0.5μCi [3H]CTP and 2μg supercoiled pGEM®-5Zf(+) Vector DNA.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

P2075, P2077, P4074 For Research Use Only. Not for Use in Diagnostic Procedures.

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