T7 RNA Polymerase

Product	Size	Conc.	Catalog #	*List Price	Order QTY	Add to Cart
T7 RNA Polymerase Protocol MSDS Components Close Window T7 RNA Polymerase  Components DTT P117B1 x 250μl Transcription Optimized 5X Buffer P118B1 x 500μl T7 RNA Polymerase P207B1 x 1,000u	1,000u	10–20u/μl	P2075	$ 67.00		Add to cart
T7 RNA Polymerase Protocol MSDS Components Close Window T7 RNA Polymerase  Components DTT P117C1 x 1.25ml Transcription Optimized 5X Buffer P118C1 x 1.25ml T7 RNA Polymerase P207E1 x 5,000u	5,000u	10–20u/μl	P2077	$ 225.00		Add to cart
T7 RNA Polymerase (HC) Protocol MSDS Components Close Window T7 RNA Polymerase (HC)  Components DTT P117B1 x 250μl Transcription Optimized 5X Buffer P118B1 x 500μl T7 RNA Polymerase (HC) P407A1 x 10,000u	10,000u	80u/μl	P4074	$ 371.00		Add to cart

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QC Tests

Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, transcription.

Source

Recombinant E. coli strain.

Storage Buffer

20mM potassium phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCI, 0.1% (v/v) Triton® X-100 and 50% (v/v) glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the incorporation of 5nmol of CTP into acid-insoluble product in 60 minutes at 37°C in a total volume of 100μl. The reaction conditions are: 40mM Tris-HCl (pH 7.9 at 25°C), 6mM MgCl₂,10mM DTT, 10mM NaCl₂, 2mM spermidine, 0.05% Tween® 20, 0.5mM each of ATP, GTP, CTP, and UTP, 0.5μCi [³H]CTP and 2μg supercoiled pGEM®-5Zf(+) Vector DNA.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

P2075, P2077, P4074 For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.