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The HDAC-Glo™ and SIRT-Glo™ Assays and Screening Systems provide a simple "Add-Mix-Measure" method that achieves maximum signal in as little as 15 minutes and is 10-to 100X more sensitive than comparable fluorescence-based systems.
Measure the relative activity of HDAC class I and II enzymes from cell extracts or purified enzyme sources with these assays and screening systems.
Selectively measure HDAC isozyme 2 activity or inhibition from cell extracts (cell-based assays) or purified enzyme sources (biochemical assays).
Selectively measure HDAC class IIa activity or inhibition from cell extracts (cell-based assays) or purified enzyme sources (biochemical assays).
Single-reagent-addition, homogeneous, luminescent assays and screening systems that measure the relative activity of the NAD+-dependent histone deacetylase class III enzymes (sirtuins; SIRTs) from purified enzyme sources.
Measure compound binding at HDAC proteins within intact cells using the NanoBRET™ technology-based target engagement assay.