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We offer a range of highly predictive, easy-to-use assays for accurate and efficient inhibitor profiling. These include bioluminescent histone deacetlyase activity assays, and convenient NanoBRET™ assays for histone:bromodomain interactions.
Live-cell protein interaction assays based on Bioluminescent Resonance Energy Transfer (BRET) for protein targets in bromodomain, transcription, and other protein families. Choose a prebuilt assay or purchase vectors and reagents to build your own assays in convenient kit format.
Measure the relative activity of HDAC class I and II enzymes from cell extracts or purified enzyme sources with these assays and screening systems.
Selectively measure HDAC isozyme 2 activity or inhibition from cell extracts (cell-based assays) or purified enzyme sources (biochemical assays).
Selectively measure HDAC class IIa activity or inhibition from cell extracts (cell-based assays) or purified enzyme sources (biochemical assays).
Single-reagent-addition, homogeneous, luminescent assays and screening systems that measure the relative activity of the NAD+-dependent histone deacetylase class III enzymes (sirtuins; SIRTs) from purified enzyme sources.
We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.
Ask us! We are here to help you.