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Promega Corporation

Histone Deacetylase Assays

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Histone deacetylase_Sirtuin Assays_9498MA
Luminescent HDAC and SIRT Assays

The HDAC-Glo™ and SIRT-Glo™ Assays and Screening Systems provide a simple "Add-Mix-Measure" method that achieves maximum signal in as little as 15 minutes and is 10-to 100X more sensitive than comparable fluorescence-based systems.

Featured Products

HDAC-Glo™ I/II Assays

Measure the relative activity of HDAC class I and II enzymes from cell extracts or purified enzyme sources with these assays and screening systems.

HDAC-Glo™ 2 Assay

Selectively measure HDAC isozyme 2 activity or inhibition from cell extracts (cell-based assays) or purified enzyme sources (biochemical assays).

HDAC-Glo™ Class IIa Assay

Selectively measure HDAC class IIa activity or inhibition from cell extracts (cell-based assays) or purified enzyme sources (biochemical assays).

SIRT-Glo™ Assays

Single-reagent-addition, homogeneous, luminescent assays and screening systems that measure the relative activity of the NAD+-dependent histone deacetylase class III enzymes (sirtuins; SIRTs) from purified enzyme sources.

Featured Resources

posters_fr A Bioluminescent HDAC Assay for Cell-Based Drug Discovery Scientific Poster

Here we describe efforts to define the utility of the HDAC-Glo™ Assay using structurally diverse HDAC inhibitors and various cell lines.

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Scientists at Your Service

Scientists at Your Service

We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.

Ask us! We are here to help you.