Our wide selection of bioluminescent enzyme substrates for proteases and metabolic enzymes such as CYPs and MAO, among others, provide powerful tools for the ADME-Tox screen.
Promega offers multiple assays to assess different mechanisms and biomarkers of apoptosis. The Caspase-Glo® 3/7, 8, and 9 Assays are the gold standards for simple apoptosis detection.
Easily measure viability in cell culture, 3D microtissues, bacterial cultures and virus-infected cells. Bioluminescent assays with simple "add-mix-measure" protocols and convenient luminescent readout allow you to easily and sensitively compare cell viability data from well-to-well, from plate-to-plate and from day-to-day.
Promega offers multiple readouts for quantifying cytotoxicity, including luminescent and fluorescent assays that can be multiplexed with apoptosis or viability assays to help determine mechanism of cell death.
Glucose is the primary energy source for mammalian cells, and NAD(P)H molecules are important cofactors for many enzymes involved in key cellular pathways. Quantitation of these two molecules can determine metabolic activity, including for cells affected by diabetes or cancer.
Caspase-1 is an essential component of the inflammasome. Conversion of the procaspase-1 zymogen into catalytically active caspase-1 results in 1) the processing and release of cytokines, IL-1β and IL-18, and 2) pyroptosis, an immunogenic form of cell death. Promega introduces a homogenous, bioluminescent method to selectively measure caspase-1 activity directly from cells or medium.
A multiplexed, cell-based assay to monitor mitochondrial health. Measures ATP and a membrane integrity biomarker to distinguish mito-toxic mechanisms from other cytotoxic events.
Promega offers assays for detecting glutathione, measuring the ratio of reduced to oxidized glutathione and detecting changes in ROS as an indicator of cell health or cell signaling events.
Reporter vectors and cell lines containing response elements for monitoring activity of key cell signaling pathways.