MAO-Glo™ Assay Systems

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Luminescent Monoamine Oxidase Assay

  • Selectively determine MAO-A and MAO-B activity in cell-based or biochemical samples
  • Luminescent assay is 100x more sensitive than fluorometric methods
  • Simple "add and read" protocol ideal for high-throughput screening

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Catalog number selected: V1401

$ 647.00
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MAO-Glo™ Assay Systems
200 assays
$ 647.00
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Selective and Sensitive Detection of Monoamine Oxidase Activity

The MAO-Glo™ Assay is performed by incubating the MAO enzyme source with a luminogenic MAO substrate. Upon reaction with MAO, the derivative is converted into luciferin, which in turn reacts with luciferase to produce light. The amount of light produced is directly proportional to the activity of MAO.

After the MAO reaction has been performed, the reconstituted Luciferin Detection Reagent is added. The reagent simultaneously stops the MAO reaction and initiates a stable glow-type luminescent signal with a half-life greater than 5 hours. This eliminates the need for strictly timed luminescent detection.

The MAO-Glo™ Assay includes a luminogenic MAO substrate, two MAO Reaction Buffers (one that can be used with either MAO A or MAO B enzyme and one that is designed specifically for MAO B), a lyophilized Luciferin Detection Reagent and the Luciferin Detection Buffer. The user supplies the sample material containing MAO. Protocols are configured for multiwell plate formats but easily can be adapted for single-tube applications.

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Conversion of the MAO Substrate by MAO. Monoamine oxidase acts on the luminogenic MAO Substrate to produce methyl ester luciferin, which is then converted into light by the esterase and luciferase enzymes in the reconstituted Luciferin Detection Reagent. Any effects of test compounds on luciferase or esterase activities have been minimized.
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MAO-Glo is >100x more sensitive than fluorometric methods. MAO-Glo provides expanded dynamic range and a more sensitive assay compared to fluorescent assay alternatives.

Measure Monoamine Oxidase Inhibitor Activity with MAO-Glo

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MAO-A (Panel A) and MAO-B (Panel B) reactions were performed in the presence of various inhibitors and the luminescent signal was measured. Results correlate with known activity of inhibitors.

Screening for MAO Inhibitors and Substrates

Monoamine oxidases process neurotransmitters (norepinephrine, serotonin, dopamine) in the brain. MAO-A and MAO-B are found in neurons and astroglia, while MAO-A is also active in the liver and other tissues. MAO dysfunction is thought to be responsible for several psychiatric and neurological disorders. An emerging research area for MAO enzymes surrounds their role in degradation of certain psychedelic treatments. MAOI (monoamine oxidase inhibitors) are a key class of antidepressant treatments and are being studied for neurodegenerative disease treatment.
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Screening for monoamine oxidase inhibitors or substrates with the luminescent MAO-Glo™ Assay System decreases the false-hit rate compared to fluorescent assays, which have a false-hit rate as high as 10%. See the data in the article The MAO-Glo™ Assay: A Bioluminescent, Coupled Assay for Monoamine Oxidase Activity.

See over 200 citations for the MAO-Glo™ Assay System.

Specifications

You are viewing: V1401 Change Configuration

What's in the box?

Item Part # Size

MAO Substrate

V141A 1 × 186μg

MAO Reaction Buffer

V142A 1 × 10ml

MAO B Reaction Buffer

V143A 1 × 10ml

Reconstitution Buffer with Esterase

V144A 1 × 10ml

Luciferin Detection Reagent

V859A 1 × 1 each

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Patent Pending.

Specifications

You are viewing: V1402 Change Configuration

What's in the box?

Item Part # Size

MAO Substrate

V141B 1 × 931μg

MAO Reaction Buffer

V142B 1 × 50ml

MAO B Reaction Buffer

V143B 1 × 50ml

Reconstitution Buffer with Esterase

V144B 1 × 50ml

Luciferin Detection Reagent

V859B 1 × 1 each

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Patent Pending.

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