ViaScript® (HiBiT) TCK Bioassay
Transfection and Cell Killing Assay in One for Optimal Target Cell Selection
- Durable: HiBiT protein is detectable as early as 8 hours post-transfection and is stable for >3 days
- Dynamic: Robust signal with as little as 2,000 cells in 96-well format or 500 cells in 384-well format
- Compatible: Pair with qualified effector cells for ADCC, ADCP and TDCC activity
ViaScript® (HiBiT) TCK Bioassay
| Catalog No. | Product Name | Size | Price | Qty | |||
|---|---|---|---|---|---|---|---|
| CS3628B19 | Early Access ViaScript® (HiBiT) TCK Bioassay | View Specifications | 1 each | Consultar Precio | |||
| CS3628B21 | Early Access ViaScript® (HiBiT) TCK Bioassay 5X | View Specifications | 1 each | Consultar Precio |
Early Access = This product is available under our Early Access program - Learn More
Catalog (FT) = This product is available under our Catalog (FT) program - Learn More
ViaScript® (HiBiT) TCK Bioassay: Screen. Select. Success.
We know how critical and frustrating it can be for pharmaceutical and biotech companies to rigorously screen cell lines and clones for target expression to ensure validity, data reproducibility and regulatory compliance. If you pick the wrong cell line, it can lead to costly experimental failures and misleading data.
However, you don’t need to spend weeks selecting a stable clone that meets the necessary criteria to move forward, which can be time-consuming, labor intensive and constrained by the variability and limitations of traditional assays. To address those limitations and expand beyond the low-throughput use of stable clones, we developed a novel mRNA transfection reagent that enables rapid and uniform expression of HaloTag-HiBiT in a wide range of target cell types.
What ViaScript® (HiBiT) TCK Bioassay does for you:
- Streamlines your cell line screening process with a high-throughput assay
- Provides a consistent, reproducible HaloTag-HiBiT mRNA transfection and target cell killing measurement that you can rely on
- Offers a simplified workflow: Pair ViaScript® HiBiT transfection with the Target Cell Killing platform for easy comparison across cell lines
Harness the Power of HiBiT in Your Target Cell Lines
Principle of ViaScript® (HiBiT) TCK Bioassay. The ViaScript® Reagent allows researchers to transiently transfect HiBiT mRNA into a cell line of interest to enable early screen and clone selection. The HiBiT cell line can then be used in a HiBiT TCK Bioassay and paired with qualified primary effector cells and a biologic to measure ADCC, ADCP, TDCC or CAR-T cell killing. Killing of the target cell releases HiBiT protein into the media, and substrate addition allows HiBiT to bind with high affinity to its LgBiT counterpart to form the functional NanoBiT® luciferase. The resulting luminescent signal is proportional to the number of dead cells in the culture and is specific to target cell death.
ViaScript® (HiBiT) TCK Bioassay for Cell Line Optimization
ViaScript® (HiBiT) TCK Bioassay approach applied to a breast cancer panel. Day 1, transfection of HaloTag-HiBiT mRNA using ViaScript® Reagent. Day 2, mixing of CD8+ T cells with target cells (10:1 E:T ratio) in the presence of varying concentrations of Ertumaxomab (HER2 X CD3). Day 3, addition of Bio-Glo-NB™ TCK Reagent followed by luminescence measurement. HER2 expression (highest to lowest) is as follows: SKBR3, MCF7, MDA-MB-231, MDA-MB468 (HER2 negative).
ViaScript® (HiBiT) TCK Bioassay enables screening of multiple target cell types simultaneously to dramatically reduce your workload.
Curious about what kinds of primary cells can you pair with ViaScript® (HiBiT) TCK Bioassay?
ViaScript® (HiBiT) TCK Bioassay is ideal for pairing with MoA-qualified primary effector cells that include:
PBMCs for ADCC, macrophage for ADCP, and CD8+ T cells for TDCC activity.
Learn more about Promega Thaw and Use Primary Effector Cells.
Protocols
No protocols available
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación | Concentración |
|---|---|---|---|
HT-HiBiT mRNA |
CS3628B17 | 1 × 20μl | |
eGFP mRNA |
EV495A | 1 × 100μl | |
ViaScript® Buffer |
EV100A | 1 × 11ml | |
ViaScript® Reagent |
EV496A | 1 × 0.1ml | 1mg/ml |
Bio-Glo-NB™ TCK Luciferase Assay Substrate |
JB100A | 2 × 0.2ml | |
Bio-Glo-NB™ TCK Luciferase Assay Buffer |
JB101A | 2 × 10ml | |
Bio-Glo-NB™ TCK LgBiT Protein |
JB102A | 2 × 0.1ml |
SDS
Search for SDSCertificado de Análisis
Contenido
| Item | Part # | Presentación | Concentración |
|---|---|---|---|
HT-HiBiT mRNA |
CS3628B17 | 5 × 20μl | |
eGFP mRNA |
EV495A | 5 × 100μl | |
ViaScript® Buffer |
EV100A | 5 × 11ml | |
ViaScript® Reagent |
EV496A | 5 × 0.1ml | 1mg/ml |
Bio-Glo-NB™ TCK Luciferase Substrate |
JB100B | 2 × 1ml | |
Bio-Glo-NB™ TCK Luciferase Assay Buffer |
JB101B | 1 × 100ml | |
Bio-Glo-NB™ TCK LgBiT Protein |
JB102B | 1 × 1ml |
SDS
Search for SDSCertificado de Análisis
Resources
Featured Resource
The ViaScript® Target Cell Killing Bioassay provides uniform, highly titratable expression and use of HiBiT TCK cell lines to create a versatile platform for cell therapy cytotoxicity assays.
- Compare potency and efficacy using panels of disease-relevant cell lines.
- Pick a cell background for stable expression—compatible with a broad range of adherent and suspension cell types.
- Compare potency and efficacy at varying levels of target expression using the same cell background.
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