MagneHis™ Protein Purification System

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Magnetic Purification of Polyhistidine- or HQ-Tagged Proteins from E. coli or Mammalian Cell Lysates

  • No centrifugation or vacuum required after cell lysis
  • Particles are compatible with a variety of common buffers
  • Binding capacity up to 1mg of polyhistidine-tagged protein per 1ml of particles

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Catalog number selected: V8500

$ 377.00
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MagneHis™ Protein Purification System
65 reactions
$ 377.00
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Purify Polyhistidine (His-) or HQ-Tagged Proteins

MagneHis™ Protein Purification System provides a simple, rapid, reliable method for the purification of polyhistidine- or HQ-tagged, expressed proteins. Paramagnetic precharged nickel particles (MagneHis™ Ni-Particles) are used to isolate polyhistidine- or HQ-tagged protein directly from a crude cell lysate using either a manual (requires use of a magnetic stand) or automated procedure. Samples can be processed in a high-throughput manner using a robotic platform such as the Beckman Coulter Biomek® 2000 or FX or Tecan Genesis® RSP.

MagneHis™ Ni-Particles are compatible with a variety of common buffers. Binding capacity is approximately 1mg of polyhistidine-tagged protein per 1ml of MagneHis™ Ni-Particles.

Note: This product requires the use of a magnetic stand.

For more information, see the Protocols & Applications Guide.

Applications
  • Protein purification of polyhistidine- or HQ-tagged, expressed proteins from a crude E. coli cell lysate.
  • High-throughput purification of His- or HQ-tagged proteins using automated platforms.
  • Purification of His-tagged proteins from insect or mammalian cell lysates.
  • Pull-down of His-tagged proteins for protein:protein interaction studies.
Purification of His-tagged methionyl tRNA synthetase.

Purification of His-tagged methionyl tRNA synthetase.

Especificaciones

You are viewing: V8500 Cambiar Configuración

Contenido

Item Part # Presentación Available Separately

MagneHis™ Binding/Wash Buffer

V851A 1 × 50ml

MagneHis™ Elution Buffer

V852A 1 × 10ml

MagneHis™ Ni-Particles

V854A 1 × 2ml View Product

FastBreak™ Cell Lysis Reagent, 10X

V857A 1 × 15ml View Product

DNase I (lyophilized)

Z358B 1 × 1 vial View Product

Certificado de Análisis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

CC

Patentes y Exclusiones

U.S. Pat. Nos. 7,112,552 and 7,354,750.

This product is licensed for use under U.S. Pat. No. 6,174,704.

Especificaciones

You are viewing: V8550 Cambiar Configuración

Contenido

Item Part # Presentación Available Separately

MagneHis™ Binding/Wash Buffer

V851B 1 × 250ml

MagneHis™ Elution Buffer

V852B 1 × 50ml

MagneHis™ Ni-Particles

V854B 1 × 10ml View Product

FastBreak™ Cell Lysis Reagent, 10X

V857B 1 × 60ml View Product

DNase I (lyophilized)

Z358A 2 × 1 vial View Product

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

CC

Patentes y Exclusiones

U.S. Pat. Nos. 7,112,552 and 7,354,750.

This product is licensed for use under U.S. Pat. No. 6,174,704.

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Uwe

Uwe

Germany