pBiT3.1 HiBiT MCS Vectors
Traditional Cloning Vectors for Generating HiBiT-Tagged Proteins
- Add an N- or C-terminal HiBiT tag to your protein of interest
- Generate an N-terminal HiBiT-tagged transmembrane or secreted protein
- Use for expression analysis and protein quantitation using luminescent detection reagents
Vectors
| Catalog No. | Product Name | Size | Price | Qty | |||
|---|---|---|---|---|---|---|---|
| N2361 | pBiT3.1-N[CMV/HiBiT/Blast] Vector | View Specifications | 20μg | List Price: $ 498.00 Your price: Acceder | Add to Cart | ||
| N2371 | pBiT3.1-C[CMV/HiBiT/Blast] Vector | View Specifications | 20μg | List Price: $ 498.00 Your price: Acceder | Add to Cart | ||
| N2381 | pBiT3.1-secN[CMV/HiBiT/Blast] Vector | View Specifications | 20μg | List Price: $ 498.00 Your price: Acceder | Add to Cart |
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The three pBiT3.1 HiBiT Vectors are configured to append the 11 amino acid HiBiT peptide tag to the amino or carboxy terminus of the target protein. These vector contain a multiple cloning region to generate an in-frame HiBiT fusion protein. The pBiT3.1 Vectors can be used for both stable and transient gene expression and encode kanamycin resistance for bacterial selection and blasticidin resistance for mammalian selection. The flexible linker between the protein of interest and the HiBiT tag will vary in length, depending on the restriction enzyme used.
The pBiT3.1-N [CMV/HiBiT/Blast] Vector appends the HiBiT tag to the N terminus of the gene of interest. The insert should contain a stop codon at the 3´ end for termination of the translation.
The pBiT3.1-C [CMV/HiBiT/Blast] Vector adds the HiBiT tag to the C terminus of the gene of interest. Note: The insert should not encode a stop codon, and the gene of interest should contain proper translation initiation sequences, including an N-terminal ATG codon or Kozak sequence.
The pBiT3.1-secN [CMV/HiBiT/Blast] Vector attaches the HiBiT tag to the N terminus of the mature form of transmembrane or secreted proteins. This vector encodes the IL-6 secretion signal peptide N-terminal to the HiBiT tag for direct trafficking of HiBiT-tagged proteins to the plasma membrane of mammalian cells for cell surface expression or secretion.
Note: The insert should also contain a stop codon at the 3´ end for termination of the translation.
The HiBiT peptide tag, in combination with the Nano-Glo® HiBiT detection systems, offers bioluminescent detection of the protein of interest. This results in quantitation of proteins using bioluminescence with no need for antibodies.
Notes:
- Expression of the HiBiT-tagged protein will only occur when the proper reading frame is maintained between the HiBiT tag and the gene of interest.
- The HiBiT peptide sequence is provided for reference only. To obtain rights to synthesize the HiBiT tag, please see the Terms and Conditions of Use.
pBiT3.1-N [CMV/HiBiT/Blast] Vector sequence text file.
pBiT3.1-C [CMV/HiBiT/Blast] Vector sequence text file.
pBiT3.1-secN [CMV/HiBiT/Blast] Vector sequence text file.
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación | Concentración |
|---|---|---|---|
pBiT3.1-N[CMV/HiBiT/Blast] Vector |
N236A | 1 × 20μg | 1μg/μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
No transfer of this product is allowed. Researchers may use this product in their own research and they may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to recipients and recipients agree to be bound by the terms of this label license.
Researchers shall have no right to modify or otherwise create variations of the nucleotide sequences of the subunits of NanoBiT® Technology, except that researchers may create fused gene sequences at the termini of the subunits of NanoBiT® Technology. No other use of this product or its derivatives is authorized without the prior express written consent of Promega.
In addition, researchers must either:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product and its derivatives, or
(1b) contact Promega to obtain a license for use of the product and its derivatives with LARs not manufactured by Promega.
For uses of Nano-Glo®-branded LARs intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must either:
(2a) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product and its derivatives, or
(2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega.
Researcher has no right to modify, derivatize, genetically engineer or otherwise create variations of the NanoBiT® Control Vectors, except that researcher may propagate and store NanoBiT® Control Vectors for use in NanoBiT® assays. With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 9,797,890 and 10,648,971; Japanese Pat. Nos. 7270842 and 7532562; and other patents and patents pending.
Contenido
| Item | Part # | Presentación | Concentración |
|---|---|---|---|
pBiT3.1-C[CMV/HiBiT/Blast] Vector |
N237A | 1 × 20μg | 1μg/μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
No transfer of this product is allowed. Researchers may use this product in their own research and they may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to recipients and recipients agree to be bound by the terms of this label license.
Researchers shall have no right to modify or otherwise create variations of the nucleotide sequences of the subunits of NanoBiT® Technology, except that researchers may create fused gene sequences at the termini of the subunits of NanoBiT® Technology. No other use of this product or its derivatives is authorized without the prior express written consent of Promega.
In addition, researchers must either:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product and its derivatives, or
(1b) contact Promega to obtain a license for use of the product and its derivatives with LARs not manufactured by Promega.
For uses of Nano-Glo®-branded LARs intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must either:
(2a) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product and its derivatives, or
(2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega.
Researcher has no right to modify, derivatize, genetically engineer or otherwise create variations of the NanoBiT® Control Vectors, except that researcher may propagate and store NanoBiT® Control Vectors for use in NanoBiT® assays. With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 9,797,890 and 10,648,971; Japanese Pat. Nos. 7270842 and 7532562; and other patents and patents pending.
Contenido
| Item | Part # | Presentación | Concentración |
|---|---|---|---|
pBiT3.1-secN[CMV/HiBiT/Blast] Vector |
N238A | 1 × 20μg | 1μg/μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
No transfer of this product is allowed. Researchers may use this product in their own research and they may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to recipients and recipients agree to be bound by the terms of this label license.
Researchers shall have no right to modify or otherwise create variations of the nucleotide sequences of the subunits of NanoBiT® Technology, except that researchers may create fused gene sequences at the termini of the subunits of NanoBiT® Technology. No other use of this product or its derivatives is authorized without the prior express written consent of Promega.
In addition, researchers must either:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product and its derivatives, or
(1b) contact Promega to obtain a license for use of the product and its derivatives with LARs not manufactured by Promega.
For uses of Nano-Glo®-branded LARs intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must either:
(2a) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product and its derivatives, or
(2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega.
Researcher has no right to modify, derivatize, genetically engineer or otherwise create variations of the NanoBiT® Control Vectors, except that researcher may propagate and store NanoBiT® Control Vectors for use in NanoBiT® assays. With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 9,797,890 and 10,648,971; Japanese Pat. Nos. 7270842 and 7532562; and other patents and patents pending.
Resources
No related resources available
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