GoTaq® G2 Hot Start Taq Polymerase
High-Performance Hot Start Taq with Convenient Room-Temperature PCR Setup
- Hot-start Taq eliminates nonspecific PCR amplification
- Detects low-copy-number targets
- Allows optimization of reactions with Flexi Reaction Buffer and separate MgCl2
- Supplied at a concentration of 5u/μl
Catalog Number:
Size
Catalog Number: M7401
Catalog Number: M7405
Catalog Number: M7406
Catalog Number: M7408
Hot Start Taq with Superior Performance, Improved Yield, Greater Sensitivity
GoTaq® G2 Hot Start Taq is the second generation of GoTaq® products. It comes in a variety of formats designed to provide maximum flexibility, control and convenience.
GoTaq® G2 Hot Start Taq is bound to a proprietary antibody that blocks polymerase activity until the reaction is heated to 94–95°C during initial denaturation. Polymerase activity is inhibited at temperatures below 70°C, allowing convenient, room-temperature reaction setup. Hot-start Taq is advantageous for some amplification targets because it may eliminate or minimize formation of primer-dimer or nonspecific products. In some cases, hot-start PCR may improve yields.
GoTaq® G2 Hot Start Taq is available as a master mix or as a standalone enzyme, it is supplied with 5X Green GoTaq® Flexi Buffer, 5X Colorless GoTaq® Flexi Buffer and 25mM MgCl2. GoTaq® G2 Hot Start Polymerase exhibits 5´→3´ exonuclease activity.
Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.
GoTaq® G2 Hot Start Taq exhibits higher yields and greater detection sensitivity. The 2.2kb APC target was amplified from decreasing amounts of input gDNA (33ng, 3.3ng and 330pg, left to right for each enzyme) using GoTaq® G2 Hot Start Taq with Green (G) and Colorless (C) buffers and the leading competitors (L1, R, Q, Q+, L2 and L3) according to manufacturers’ recommendations.
Protocols
Complete Protocol
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación | Concentración | Available Separately |
|---|---|---|---|---|
|
MgCl2 |
A351B | 1 × 750μl | 25mM | View Product |
GoTaq® G2 Hot Start Polymerase |
M740A | 1 × 100u | ||
|
5X Colorless GoTaq® Flexi Buffer |
M890A | 1 × 1ml | View Product | |
|
5X Green GoTaq® Flexi Buffer |
M891A | 1 × 1ml | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Concentración | Available Separately |
|---|---|---|---|---|
|
MgCl2 |
A351H | 3 × 1.2ml | 25mM | View Product |
GoTaq® G2 Hot Start Polymerase |
M740B | 1 × 500u | ||
|
5X Colorless GoTaq® Flexi Buffer |
M890A | 4 × 1ml | View Product | |
|
5X Green GoTaq® Flexi Buffer |
M891A | 4 × 1ml | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Concentración | Available Separately |
|---|---|---|---|---|
|
MgCl2 |
A351H | 15 × 1.2ml | 25mM | View Product |
GoTaq® G2 Hot Start Polymerase |
M740B | 5 × 500u | ||
|
5X Colorless GoTaq® Flexi Buffer |
M890A | 20 × 1ml | View Product | |
|
5X Green GoTaq® Flexi Buffer |
M891A | 20 × 1ml | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Concentración | Available Separately |
|---|---|---|---|---|
|
MgCl2 |
A351H | 60 × 1.2ml | 25mM | View Product |
GoTaq® G2 Hot Start Polymerase |
M740B | 20 × 500u | ||
|
5X Colorless GoTaq® Flexi Buffer |
M890A | 80 × 1ml | View Product | |
|
5X Green GoTaq® Flexi Buffer |
M891A | 80 × 1ml | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Resources
Related Products
Productos Similares
GoTaq® G2 Hot Start Master Mixes
Master mix optimizada lista para su uso en la preparación de PCR a temperatura ambiente. Contiene la enzima GoTaq® G2.
M7422, M7423, M7432, M7433
GoTaq® G2 DNA Polymerase
GoTaq® Polymerase de última generación: alto rendimiento para sus necesidades diarias de PCR.
M7841, M7845, M7848
GoTaq® G2 Flexi DNA Polymerase
GoTaq® G2 DNA Polymerase de segunda generación y alto rendimiento con tampones sin Mg.
M7801, M7805, M7806, M7808
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