ReliaPrep™ miRNA Cell and Tissue Miniprep System
Purify concentrated RNA quickly and safely
The ReliaPrep™ miRNA Cell and Tissue Miniprep System isolates total RNA including microRNA (miRNA) and other small non-coding RNA (sncRNA) subspecies from a variety of cell and tissue types. The system reliably and consistently purifies RNA from as few as 100 to as many as 1 × 106 cultured cells, and from 0.25 to 20mg of tissue. Purified RNA is extracted in a low elution volume (as low as 15μl) ready for use in your downstream assays.
Organic-based purification has been accepted as the most sensitive method to purify RNA. The ReliaPrep™ miRNA purification system achieves virtually the same yield and purity of amplifiable miRNA without phenol:chloroform extraction, eliminating the need to handle and dispose of harsh organic products.
Purified, concentrated RNA includes miRNA and subspecies
RNA Species from Cells
Expression of miRNA (miR16), snoRNA (U6), and sncRNA (RNU44 and RNU48) isolated from HEK293 cells.
RNA Species from Tissue
Expression of miRNA and snoRNA products detected from total RNA isolated from 10mg of frozen mouse liver and kidney tissue.
RNA can be purified from small sample sizes
The ReliaPrep column/binding matrix efficiently captures RNA from very small amounts of input material.
RNA from 10–105cells
Here, the miRNA targets U6 and miR16 were amplified after RNA isolation from varying cell culture inputs. RNA was purified from as few as 10 cells to as many as 1x105 HEK293 cells. miR16 and U6 expression levels were determined using qPCR.
RNA from 0.0032–10mg Tissue
In this example, the miRNA targets let7e and miR16 were reliably amplified from ReliaPrep-purified RNA isolated from 0.0032–10mg frozen mouse kidney tissue. miR16 and let7e expression levels were determined by qPCR.
Isolate pure RNA without phenol:chloroform extraction
The ReliaPrep™ miRNA System does not use phenol chloroform yet results in acceptably pure RNA isolated from a range of tissue types. The data below show purity rations for RNA purified from various mouse tissues using the ReliaPrep™ miRNA Cell and Tissue Miniprep System and other commercial miRNA purification kits.
RNA purity A260/A280
All kits purified RNA with A260/A280 purity ratios in the accepted 1.8–2.2 range.
RNA purity A260/A230 ratios
Kits using phenol chloroform reagents generated RNA with A260/A230 ratios below the accepted 1.8–2.2 range.
Download the poster
MicroRNA Analysis Paired with a Novel Live Cell Viability Assay: A Complete Epigenetic Workflow in Human Cancer Cell Lines
In this poster, the authors used total RNA, mRNA and miRNA isolated with the Reliaprep™ miRNA system to examine the role of small or non-coding RNAs in cancer cell growth and metastasis.
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
Collection Tubes |
A130C | 2 × 10/pk | |
| ReliaPrep™ Minicolumns | A207A | 2 × 10/pack | |
| 1-Thioglycerol | A208A | 2 × 75μl | |
DNase Buffer, 10X |
M198B | 1 × 500μl | |
|
Nuclease-Free Water |
P119A | 1 × 1,250μl | View Product |
Lysis Buffer (LBA) |
Z106A | 1 × 6ml | |
|
Elution Tubes |
Z200B | 1 × 25/pk | |
|
RNA Wash Solution (RWA) |
Z309B | 1 × 11.8ml | View Product |
|
DNase I (lyophilized) |
Z358B | 1 × 1 vial | View Product |
|
RNA Dilution Buffer (RDB) |
Z372C | 1 × 9ml |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
Collection Tubes |
A130A | 2 × 50/pk | |
ReliaPrep™ Minicolumns |
A207B | 2 × 50/pack | |
|
1-Thioglycerol |
A208B | 1 × 900μl | |
DNase Buffer, 10X |
M198B | 1 × 500μl | |
|
Nuclease-Free Water |
P119E | 1 × 13ml | View Product |
Lysis Buffer (LBA) |
Z106B | 1 × 30ml | |
|
Elution Tubes |
Z200C | 2 × 50/pk | |
|
RNA Wash Solution (RWA) |
Z3091 | 1 × 58.8ml | View Product |
|
DNase I (lyophilized) |
Z358A | 1 × 1 vial | View Product |
|
RNA Dilution Buffer (RDB) |
Z372A | 1 × 44ml |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
Collection Tubes |
A130B | 2 × 250/pk | |
ReliaPrep™ Minicolumns |
A207B | 10 × 50/pack | |
|
1-Thioglycerol |
A208B | 4 × 900μl | |
DNase Buffer, 10X |
M198B | 3 × 500μl | |
|
Nuclease-Free Water |
P119C | 1 × 25ml | View Product |
Lysis Buffer (LBA) |
Z106C | 1 × 150ml | |
|
Elution Tubes |
Z200C | 10 × 50/pk | |
|
RNA Wash Solution (RWA) |
Z309D | 1 × 206ml | View Product |
|
DNase I (lyophilized) |
Z358A | 5 × 1 vial | View Product |
|
RNA Dilution Buffer (RDB) |
Z372B | 1 × 220ml |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Resources
Artículos
- RNA Isolation from 3D Microtissue Cultures: A Comparison of Manual and Automated Methods
- microRNA Purification from Plant Tissues Using the ReliaPrep™ miRNA Cell and Tissue Miniprep System
- Total RNA Isolation from 3D Cell Cultures or Cells in Matrigel® Matrix Using the ReliaPrep™ miRNA Cell and Tissue Miniprep System
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