Wizard® Plus Maxipreps DNA Purification System
300µg–1mg Plasmid DNA from 100–500ml Cultures
- 3-hour protocol
- Resin-based batch-binding and column-washing method
- No organic extractions
Catalog Number:
Size
Catalog Number: A7270
The Wizard® Plus Maxipreps DNA Purification System provides a rapid resin-based batch column method for purification of plasmid DNA that eliminates the need for cesium chloride:ethidium bromide gradient centrifugation. Use of this system requires only a centrifuge, a vacuum source and a vacuum manifold. This system typically yields up to 500μg of high-copy-number plasmid DNA (200–20,000bp) from a 100–500ml culture in less than three hours. The purified DNA is eluted in Nuclease-Free Water (Cat.# P1193) and can be used directly for DNA sequencing and restriction digestion without further manipulation. The DNA also can be used for in vitro transcription reactions supplemented with a ribonuclease inhibitor such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511).
Protocols
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
Cell Resuspension Solution |
A711C | 1 × 150ml | View Product |
|
Cell Lysis Solution |
A712D | 1 × 150ml | View Product |
|
Neutralization Solution |
A713C | 2 × 150ml | View Product |
|
Maxi/Megapreps Filtering System |
A742D | 2 × 5 pack | View Product |
|
Wizard® Maxipreps DNA Purification Resin |
A769A | 1 × 100ml | View Product |
|
Column Wash Solution |
A810C | 1 × 125ml | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
U.S. Pat. No. 5,808,041.
Resources
No related resources available
Related Products
Productos Similares
PureYield™ Plasmid Miniprep System
Hasta 15μg de plásmido a partir de cultivos de 3ml, listo para la transfección en 10 minutos.
A1223, A1222
PureYield™ Plasmid Maxiprep System
1mg de plásmido a partir de cultivos de 250ml, listo para la transfección en 1 hora.
A2392, A2393
PureYield™ Plasmid Midiprep System
Hasta 200µg de plásmido a partir de cultivos de 50 ml, listo para la transfección en 30 minutos.
A2492, A2495, A2496
Wizard® Plus SV Minipreps DNA Purification Systems
Métodos de centrifugación y de vacío para la purificación de plásmidos a partir de cultivos de 1-10 ml.
A1330, A1460, A1465, A1340, A1470
Usado con frecuencia junto con
pGEM®-T Vector Systems
Vector T para la clonación simplificada de productos PCR.
A3600, A3610
Vac-Man® Laboratory Vacuum Manifold
Para una purificación de ácidos nucleicos rápida y eficaz. Procesa hasta 20 muestras de forma simultánea.
A7231
Vac-Man® Jr. Laboratory Vacuum Manifold
Para una purificación de ácidos nucleicos rápida y eficaz. Procesa dos muestras a la vez.
A7660, A3110
Nuclease-Free Water
Calidad probada y certificada libre de actividad DNasa y RNasa.
MC1191, P1193, P1195, P1197, P1199
Cell Resuspension Solution
Component of several plasmid DNA purification systems available for separate purchase.
A7112, A7113, A7114, A7115, A7118
Neutralization Solution
Component of several plasmid DNA purification systems available for separate purchase.
A7131, A7132, A1485, A1488
Cell Lysis Solution
Component of several plasmid DNA purification systems available for separate purchase.
A7122, A7123, A7124, A7125, A7128
