Trypsin/Lys-C Mix, Mass Spec Grade

V5072_Trypsin-3aLys-C-Mix--Mass-Spec-Grade_3
Protein Cleavage with Trypsin-Lys-C
V5072_Trypsin-3aLys-C-Mix--Mass-Spec-Grade_3
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Improve Protein Digestion with Mix of Trypsin Gold and rLys-C

  • Standard overnight digestion, nondenaturing conditions
  • Increased peptide recovery, enhanced protein quantitation, improved reproducibility
  • Tolerant to trypsin-inhibiting contaminants

Size

Catalog number selected: V5071

$ 77.00
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This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
Trypsin/Lys-C Mix, Mass Spec Grade
20μg
$ 77.00
Your price: Acceder
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Trypsin/Lys-C Mix, Mass Spec Grade: See What You've Been Missing

Using standard digestion conditions, Trypsin/Lys-C Mix improves peptide digestion over trypsin alone, increasing peptide recovery and resulting in better protein quantitation.
Trypsin digest illustration.

Increased Peptide and Protein Identifications Using a Standard Trypsin Digestion Protocol

Standard overnight trypsin digest schematic 11788ME-W-a
Trypsin LysC overnight digest schematic 11788ME-W-b
Data comparing trypsin and Trypsin/Lys-C digests.
Incomplete digestion with trypsin was evident after comparing peptide peak intensities in trypsin and Trypsin/Lys-C Mix digests.

Improved Reproducibility using Trypsin/Lys-C

Reproducible IgG (Rituximab) digestion with Trypsin Lys-C mix.
Improved reproducibility of IgG (Rituximab) digestion when using the Trypsin/Lys-C Mix.

Tolerant To Trypsin-Inhibiting Contaminants

Trypsin LysC digestion results in fewer missed cleavages. 13058MC-W-a
Trypsin LysC increases protein identification compared to trypsin alone. 13058MC-W-b
Efficient digestion with Trypsin/Lys-C Mix in the presence of trypsin-inhibiting agents. Yeast protein extract was used as a model system.
Conclusions: Use of Trypsin/Lys-C resulted in fewer missed cleavages and a 20% increase in protein identifications.

Protocolos

Complete Protocol

Especificaciones

You are viewing: V5071 Cambiar Configuración

Contenido

Item Part # Presentación

Resuspension Buffer

 V181A 1 × 500μl

Trypsin/Lys-C Mix, Mass Spec Grade

 V507A 1 × 20μg

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

BB

Especificaciones

You are viewing: V5072 Cambiar Configuración

Contenido

Item Part # Presentación

Resuspension Buffer

 V181A 1 × 500μl

Trypsin/Lys-C Mix, Mass Spec Grade

 V507B 1 × 100μg

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

BB

Especificaciones

You are viewing: V5073 Cambiar Configuración

Contenido

Item Part # Presentación

Resuspension Buffer

 V181A 1 × 500μl

Trypsin/Lys-C Mix, Mass Spec Grade

 V507A 5 × 20μg

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

BB

Recursos

Posters

Otros

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Usado con frecuencia junto con

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Sitio de escisión: N-terminal de Asp. pH óptimo: 4-9.

V1621

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Sitios de escisión: C-terminal de Tyr, Phe, Trp. pH óptimo: 7-9.

V1061, V1062

Encontremos el producto que se adapte a sus necesidades.

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Robert

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