6 x 5 LC-MS/MS Peptide Reference Mix
Peptide Mix and Free Software to Monitor LC/MS Instrument Performance
- Determine mass spec instrument sensitivity and dynamic range
- No manual calculations, maximized instrument performance with free PReMiS™ Software
Catalog Number:
Size
Catalog Number: V7491
Catalog Number: V7495
A Complete Solution for Monitoring LC and MS Instrument Quality Control and Performance
In combination with an accompanying software tool, the 6 × 5 LC-MS/MS Peptide Reference Mix provides a convenient way to assess LC column performance and MS instrument parameters, including sensitivity and dynamic range.
- Consists of 30 peptides: 6 sets of 5 isotopologues of the same peptide sequence, differing only in the number of stable, heavy-labeled amino acids incorporated into the sequence.
- Labels are uniform 13C and 15N atoms.
- Isotopologues are chromatographically indistinguishable; however, since they differ in mass, they are clearly resolved by mass spectrometry.
- The isotopologues of each peptide are present in a series of tenfold dilutions. This format allows assessment of instrument dynamic range and sensitivity from a single run.
The 6 × 5 LC-MS/MS Peptides are AAA-qualified for accurate monitoring, and are compatible with multiple applications and neat or complex mixtures.
Read an ACS Analytical Chemistry article by J. Beri et al. featuring use of the 6 x 5 Reagent: Reagent for Evaluating Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) Performance in Bottom-Up Proteomic Experiments.
Workflow with PReMiS™ Software
The complimentary PReMiS™ Software streamlines routine analysis by:
- Eliminating time-consuming manual calculations
- Ensuring consistent instrument performance over time
- Allowing you to compare instrument-to-instrument performance over time
Compatible with Neat and Complex Mixtures
- Use neat format for standard instrument monitoring
- Spike peptide mix into complex samples (i.e., human cell extract) to monitor instrument performance with relevant experimental samples
| Parameter/Feature | Category | Calculated by Software | Details |
|---|---|---|---|
|
Mass Accuracy
|
MS
|
Yes
|
—
|
|
Performance Over Time
|
Both
|
Yes
|
Parameters like LC retention time, peak height and mass accuracy are graphically displayed based on date and time.
|
|
Multi-Instrument Comparisons
|
Both
|
Yes
|
—
|
|
Retention Time
|
LC
|
Yes
|
Time required for peptide being separated to elute from the chromatography column.
|
|
Peak Width
|
LC
|
Yes
|
Peak width is an indication of column efficiency.
|
|
Base Peak Height
|
LC
|
Yes
|
This measure of the signal response can be correlated to the ionization efficiency of the peptide.
|
|
Graphical XIC Analysis
|
LC
|
Yes
|
A graphical XIC (eXtracted Ion Chromatogram) is displayed for all 30 ions for visual confirmation of the detectability of all masses. This is especially important for analysis of the peptide mixture spiked into a complex background.
|
|
Lowest Detectable Quantity
|
MS
|
Yes
|
Lowest abundance detected is reported for each peptide sequence.
|
|
Linear Fit Analysis (Linear Dynamic Range1)
|
MS
|
Yes
|
*The software does not directly calculate the full dynamic range of the instrument. However, up to 5 logs of dynamic range can be determined directly from the Linear Fit Analysis.
|
|
LOD and LOQ2
|
MS
|
No
|
The AAA-qualified peptides allow the mix to confirm instrument LOD and LOQ, the basis for the amount loaded, which then can be used to confirm consistency of detection limits.
|
Protocols
Complete Protocol
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación | Concentración |
|---|---|---|---|
6 × 5 LC-MS/MS Peptide Reference Mix |
V749A | 1 × 50μl | 0.5pmol/μl |
Aliquot Tubes |
V750A | 1 × 6 each |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
U.S. Pat. Nos. 10,247,711 and 11,692,983; European Pat. No. 2956768; and other patents pending.
Contenido
| Item | Part # | Presentación |
|---|---|---|
6 × 5 LC-MS/MS Peptide Reference Mix |
V749B | 1 × 200pmol |
Aliquot Tubes |
V750A | 1 × 6 each |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
U.S. Pat. Nos. 10,247,711 and 11,692,983; European Pat. No. 2956768; and other patents pending.
Resources
Software y Firmware
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