ViviRen™ Live Cell Substrate
Sensitive Quantitation of <i>Renilla</i> Luminescence in Live Cells
- Engineered coelenterazine for live-cell reporter gene, BRET and RNAi assays
- Permits real-time measurement in multiwell plates
- High signal-to-background ratios
Catalog Number:
Size
Catalog Number: E6491
Catalog Number: E6492
Catalog Number: E6495
Generate Kinetic Profiles for Reporter Assays, BRET and RNAi in Living Cells
ViviRen™ Live Cell Substrate is a uniquely engineered coelenterazine that generates three- to fivefold brighter Renilla luciferase luminescence than wildtype coelenterazine. Using live cells, achieve up to 100-fold higher signal-to-noise ratios for super-sensitive quantitation of reporter gene, BRET and RNAi activity.
Cat.# E6491 is supplied as a liquid, 60mM in DMSO. Cat.# E6492 and E6495 are each supplied as a lyophilized solid.
Assay Advantages
- Quantitate with confidence using miniaturized formats, low-level expression and CCD imagers.
- Unparalleled sensitivity with up to 100-fold higher signal-to-noise ratios than coelenterazine.
- Multiplex with CellTiter-Glo® and other lytic assays for improved accuracy and precision.
Luminescence reaches near-maximum levels within minutes of ViviRen™ Live Cell Substrate or coelenterazine addition. CHO cells stably expressing Renilla luciferase were exposed to 20µM ViviRen™ Live Cell Substrate or coelenterazine approximately 1.5 minutes after continuous luminescent reading was initiated. For more details, see Figure 5 of TM064.
Protocols
Complete Protocol
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación |
|---|---|---|
|
ViviRen™ Live Cell Substrate |
E649A | 1 × 0.37mg |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
See Protocol for detailed storage recommendations.
Contenido
| Item | Part # | Presentación |
|---|---|---|
|
ViviRen™ Live Cell Substrate |
E649B | 1 × 3.7mg |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
See Protocol for detailed storage recommendations.
Contenido
| Item | Part # | Presentación |
|---|---|---|
|
ViviRen™ Live Cell Substrate |
E649C | 1 × 37mg |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
See Protocol for detailed storage recommendations.
Resources
No related resources available
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